I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Distortion problem in Abaqus explicit?

Dear all. I'm modeling the RC column with cyclic displacement by Abaqus-Explicit. The concrete model used Concrete Plastic Damage model. However I'm facing with a error about the excessive...

01 February 2021 7,726 1 View

Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

12 January 2021 144 1 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

How to intepret data with significant ANOVA but not significant Tukey test?

I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...

25 November 2020 920 4 View

Acesulfame Potassium and Acesulfame and Conversion?

Dear to whom it may concern, Based on ChemAxon, I calculated pKa values of Acesulfame as follows: 1. pKa (Strongest Acidic): 3.02 2. pKa (Strongest Basic): -6 Because I would like to convert...

25 November 2020 4,577 3 View

How to determine the orders of High pass filter for ECG signal ( cutoff frequency = 0.033 Hz so near 0 Hz) ?

I'm trying to theoretically design a active High-Pass Filter for ECG signal with cutoff frequency Fc = 0.003 Hz...it's too close to 0 Hz and stop band frequency must be greater than 0 Hz . So...

18 November 2020 9,089 1 View

How to perform Cluster analysis for segmentation with two latent factors?

Hello, My name is Elena and working on cluster analysis for respondents segmentation based on two latent factors. I wonder whether I am doing the right way. I have 400 respondents answering on 2...

19 October 2020 1,697 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

What will happen (quality or quantity wise) if we transplant 'Aerobic Rice' in puddled condition?

I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?

08 February 2021 2,343 6 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

What are the new researchable area under Rice crop under Agronomy related to moisture stress and nanotechnology ?

I had already worked on the moisture stress and lot of works being done in rice regarding stress or drought. I want to know some very recent researchable areas in rice crop regarding moisture...

26 January 2021 5,518 7 View

ITS primers for fungal characterization?

For example, I need to characterize Rhizoctonia and Trichoderma simultaneously. In literature they are giving ITS1 &ITS3 for Trichoderma and ITS2 &ITS4 for Rhizoctonia. In our lab there is...

19 January 2021 4,157 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Is it possible to separate the momilactone molecule by GPC device?

The momilactone molecule is present in rice and has an allochemical effect on weeds.

01 January 2021 3,367 3 View

How can I use amylase enzyme to digest molluscan tissue before DNA extration?

Hi, I have a problem with a hight contet of mucopolysaccharides in some molluscan samples. I tried different DNA extration procolols but I can't already remove all the molecules that inhibit PCR...

01 January 2021 8,900 3 View