I am working in fungal fermentation and use Nexcelom omm cell counter to quantify the cells. And I am also plating them to get the cell count after 48 hours. And there is a very low correlation between them. Has anyone faced this problem?
Dear Mr. Ram Bhattarai: There will be a difference between cell counter and plating methods. The cell counter may give the results including dead cells and other suspended particles. It is always better to plate the cells for assessing live cells.
Prabakaran Krishnamurthi Thank you. Yes, there is a difference between the live cells given by the cell counter and plating.
Plating will give you a viable count - but is dependent on having a suitable dilution and adequate colonies to give a reliable count. The cell counter will give a total count and may include dead cells, though it may also miss cells that have lost cytoplasm. If there is particulate matter in your culture some may be mistaken for cells. And if your yeast are able to make pseudohyphae that will further increase the differences between the total cell count and the plated viable count. When working with filamentous fungi or yeast which are making pseudohyphae then the plate count will give a number of viable colony forming units (not individual cells) and automated cell counting is probably not possible.
- Badges
- Science method