Hi,

I have a question regarding DNA extractions.

I follow a magnetic bead based protocol for DNA extraction and at the end of the protocol, there is a step to spin down the DNA samples which are already in the elution buffer at 14,000 rpm for 10 minutes.

The rationale behind this step is to try and pellet the left over beads that carry over so that it doesn't affect the spectrophotometry when trying to quantify the DNA (A260/A280, conc. etc). And when you do spin down the tubes, you can usually see the tiny brown dot of beads.

My question is, does this spinning down also pull down the DNA or does it have no effect as the DNA will be in free suspension as its in elution?

I have attempted to test this my self by making repeat nanodrop measurements from the top layer, middle and bottom layer of the eppenforfs and found no substantial difference in the concentration but curious to see if anyone has any experience/input regarding this?

Thank you in advance.