Protocol Used

1. The slides were dipped in a trypsin jar for 15 seconds.

2. Immediately after, the slides were dipped in PBS for another 15 seconds.

3. Giemsa stain was applied, and the slides were allowed to stand for 8 minutes.

4. Finally, the slides were left to air dry.

Unfortunately, this process did not yield separated chromosomes or visible G-banding. I would greatly appreciate any advice on adjustments or suggestions for improving this protocol. Has anyone experienced similar issues or found modifications that work better?