Can someone explain about Different output of C/T vs A/G CSN1S2?

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Can I mix TRITC-labeled dextran and FITC-labeled lectin for one injection instead of having to do two injections of each?

For my protocol, at end-point I have to do tail-vein injections of TRITC-labeled dextran (100 ml of 10 mg/ml) and FITC-labeled lectin (50 mg). However, the protocol I'm following states that they...

01 May 2024 8,090 1 View

HiPSCs-derived neuroprogenitors detaching from middle -not edges, of the well in P6 plates, thoughts?

Hi all, In my lab we are currently differentiating neurons from hiPSCs according to the protocol used in Satir, T et al, 2020 (doi: 10.1038/s41598-020-57516-7). We have been successful in the...

27 March 2024 7,900 0 View

HI Mark, Please: Do you think ¿Is there any correlation between the malaria pigment and the volutin granules?

HemInozoin cristals are very compact in human parasites. However in opther haemoparasites as order haemosporidia is observed volutin. Do you think is there any correlation between protein...

26 February 2024 3,806 2 View

Can freeze-drying of fermentable product alter the results of sugar profile of the sample?

We want to conduct the sugar profile (Arabinose, Xylose, Galactose etc...) of a fermentable feedstock from different varieties. To reduce the degradation chances of this fermentable feedstock we...

10 January 2024 1,548 3 View

Do DNA diffuse from agarose gels?

how fast do DNA and RNA fragments diffuse from an agarose gel?

15 August 2023 7,142 5 View

How to remove ethanol from carbon dots solution to obtain just carbon dots?

I am synthesizing carbon dots with ethanol as the solvent. I tried to do oven drying but they stick on the wall of the glass vial. I tried to do air drying also but still the same. I tried to do...

21 June 2023 3,838 4 View

Have someone found differences in the circadian profile of Sprague-Dawley rats from different vendors?

Hi :) I am about to start a project in the circadian field and I will need to decide from now on which vendor I am going to select to purchase my animals. I would like to ask you guys if somebody...

29 May 2023 1,441 0 View

Can we neutralize concentrated Nitric acid with 1 M Phosphate buffer?

I am trying to neutralize nitric acid since the pH of my solution drops to zero after nitric acid treatment of my sample.

18 May 2023 6,411 3 View

"Hydrodynamic Analysis of Flow and Reduction of Local Scour in Longitudinal Walls."¿?

Dear colleagues, consultants, and builders, I hope this message finds you well. I am reaching out to request your kind cooperation and support in disseminating the following survey. This survey is...

09 May 2023 7,674 0 View

Can somebody know about CAPN13 in cattle?

Hi i am looking about references of posible asociation of CAPN13 gene and meat quality in cattle if you have information i be grateful

01 May 2023 2,728 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View