What do you think makes an effective classroom-instruction?

class management ;interaction between teachers and students;

16 November 2020 5,301 4 View

Microencapsulation of a PCM mixture containing an alcohol and fatty acid ester

I know this is a long shot but I am trying to microencapsulate a PCM containing a mixture of an alcohol and fatty acid ester and have currently only found in-situ polymerisation using the...

10 April 2017 6,123 3 View

Will a luciferase promoter activity assay work if the test cells don't endogenously express the gene of interest?

I am currently working up a set of experiments to determine the signaling pathways regulating transcriptional control of our gene of interest. We already have full length and truncated promoter...

01 June 2016 9,506 1 View

What is the decay curve of a plasmid in the nucleus of a *quiescent* cell, or of protein expression therefrom?

The half-life of a plasmid in cells in culture is fairly brief, but a lot of that is presumably because of dilution due to cell division. What happens when a plasmid has achieved entry into the...

07 March 2016 1,784 1 View

Is there an accepted minimum threshold volume for defining a storm event? When can rainfall start to be classified as a storm event?

23 February 2016 7,976 3 View

How can I express mature miRNA in microglia in vivo?

I want to directly express the mature form, but not precursor (shRNA) of miRNA in the microglia of mouse brain. Could anybody give some suggestions?

06 May 2015 7,439 2 View

Has anyone used the Nielsen Biconic Yield Criterion to model the incremental collapse of a reinforced concrete slab?

06 September 2014 3,287 1 View

How do I find references from Cryst. Struct. Commun. from the time before this term was attached to Acta Crystallographica C?

I know such publications exist from references to my own work but can not find the original articles.

06 December 2013 6,199 2 View

Which type of feeder layers do you prefer during iPSC reprogramming- irradiated mefs, mytomycin C treated mefs, and why?

Want to know the ups and downs of two feeder layer culture systems for iPSCs.

28 February 2013 3,659 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View