Which is the best kit/enzymatic method Efficient for the isolation of cells from the mouse or human tumors?

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DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

ANSYS APDL RC Beam modelling?

Hello I have modelled RC beam in ANSYS apdl. first create volume, then create keypoints & create a line joining that keypoints to make reinforcement. I am working on thermal analysis, for...

20 January 2021 7,344 2 View

ANSYS APDL tabular entry related?

Hello I want to add thermal convection load on specific area of concrete volume. it asks for film coefficient which is a constant value but the bulk temperature has no. of values that i have as...

18 January 2021 6,627 1 View

What is the best way to maintain constant concentration of a volatile organic liquid in an open experimental set-up?

The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.

27 December 2020 7,325 2 View

How i can input temperature depedent stress strain curve for concrete & steel in ansys?

i am working on thermo-mechanical analysis of RC beam using ANSYS. i want to input temperature dependant stress strain curve in ansys. i tried using elastic > multilinear but a warning attached...

23 December 2020 9,928 2 View

During transformation of Agrobacteriumu with plasmids ,PCR Positive colonies are not POSITIVE after restreaking.. Why?

Initially we are getting positive amplification .The positive colonies are selected and restreaked.The PCR is not showing positive amplification.

18 December 2020 9,802 3 View

What are some best features that can be added in IoT based pollution monitoring system ?

Actually I am developing IoT based pollution monitoring system and need some ideas which help in developing great product with great features

10 October 2020 5,657 7 View

Can LoRa Technology be used for managing underwater data center ?

As per the latest research, Microsoft finds underwater data centers are reliable, practical and use energy sustainably so how can we enable IoT with LoRa technology in the underwater data center...

05 October 2020 535 4 View

How can I calculate the concentration of nanoparticles being used in UV-visible absorbance scan?

I have taken 1000 PPM of nanoparticles in 1 ml of RO water and pursuing its UV visible spectro absorbance scan. They are showing respective peaks on the baseis of energy transition of electrons...

19 September 2020 6,276 3 View

How can Gold be extracted from mining concentrates?

I work at a precious metal refinery. We extract gold using aqua regia. However, sometimes Gold does not dissolve in AR. Sometimes it precipitates due to cold weather? Is there a way to make sure...

07 August 2020 7,059 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View