08 August 2018 2 729 Report

I want to isolate the cells from the tumors (mouse model and human). I read that Collagenase I is harsher than Collagenase IV. Can anyone please suggest if there is any commercial kit with the combination of enzymes (collagense, dispase and DNase or their alternative)? If such kit doesn't exist, please share your strategy. My goal is the integrity of receptors on the isolated cells for downstream application. Thanks.

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