I have a quite bright autofluorescence in the mouse brain in vivo. I can remove it only reducing the Gain or Laser Power, but it is appeared to be not enough to image my fluorescent dye. Especially in wavelenth aroung 600nm. I know that there are many ways to reduce this lipofuscin autofluorescence in fixed tissues, but in vivo the only known for me is to take a mouse which is young. This does not work in my case.
Hi Igor,
have you thought of filtering your signal with a dichroic mirror? You could just cut the af signal below 600nm.
Regards, erik
use CuSo4 or sudan black B
Article Schnell, S.A., Staines, W.A. & Wessendorf, M.W. Reduction of...
We haven't tried this our studies, but it has been known low fluorescence diet/mouse chow could reduce autofluorescence in abdominal and cutaneous tissues. Not sure about brain tissue.
Article Diet and abdominal autofluorescence detected by
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