Currently, I'm searching the best method to quantify Jasmonic Acid in plant tissue. Previously I have tried to analyze by using GC-MS but I failed. I am planning to try HPLC and the available HPLC that I had found is UV-detector. Can anyone who had an experience on this analysis advise me, what is the best gradient elution to apply with a mobile phase of acetonitrile and triethylamine? Or any other suggestion? Thank you in advance.
Hello colleague, good afternoon
First of all I suggest that you do not use triethylamine unless absolutely necessary, it usually causes undesirable increases in system pressure.
I recommend that if you are going to work with HPLC with a UV detector, you test a mobile phase composed of methanol-water (60:40) with 1% (V/V) acetic acid, at a flow rate of 0.8 mL min-1 using a 25 cm x 4.6 mm x 5 µm id Hypresil ODS column at a wavelength of 295 nm., then say me.
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Hi Good Afternoon Sir Jiménez Rodríguez . Thank you very much for your suggestion and helpful opinion. I really appreciate it.
Hola, buenos días
Ante todo me alegra saber nuevamente de Ud.
Por otro lado, cualquier otra duda puede consultarme, me agrada el intercambio de experiencias con colegas de diferentes instituciones.
Le añado además que el trabajo con los ácidos orgánicos de cadena corta y larga es muy interesante, versátil y sobre todo muy útil.
Le refiero, que en septiembre próximo estaré impartiendo clases prácticas de cromatografía en la Facultad de Química de la Universidad de la Habana a alumnos del cuarto año de Licenciatura en Química.
Saludos cordiales
Lic. Luis E Jiménez Rodríguez, MC
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