I have been trying to reduce the background signal in southern blot. I always get a dark stain (not resembling bands) in random places of my membrane.

I do the hybridisation step in a hybridisation tube. Do you think that putting a nylon mesh in between the membrane and the tube wall would help reduce the background? Or if you have any other suggestions how to reduce the background, I will be really grateful for those as well!

Thanks!

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