Best method to crush ticks and Isolate DNA (to detect Haemoparasite)?

More Sabir Hussain's questions See All

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

18 February 2021 6,483 3 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View

How can I calculate convective heat transfer coefficient from turbulent boundary layer properties?

I know many textbooks include empirical relations between Nu, Re_x, and Pr inside a turbulent boundary layer on a flat wall. However, I want to directly calculate h from known boundary layer...

19 January 2021 1,294 3 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

Please Guide me on this how to solve this issue ?

I have submitted a paper in a journal and the reviewers reply to me with this. Can anyone guide me on this how to solve it Thanks in advance Figure 4: (i) the image presented in panel (a) shows...

15 December 2020 636 6 View

Hello, What kind of statistical Analysis test can be used for the survey questions with four different options?

I have conducted a survey which includes two groups (1-Normal people, 2-Deaf and Dumb), and the questionnaire had a category of questions with 4 different options and to answer only tick one. I...

01 December 2020 1,147 3 View

How I can optimize tick miRNA RT-qPCR ?

Dear researchers, I have ordered customized primers for ticks candidate miRNAs as these miRNAs are not available in any company. I have used a miRNA-specific MystiCq cDNA synthesis kit for cDNA...

14 October 2020 5,672 7 View

How to tie master nodes to slaves nodes of a rectangular structure to indicate a complete disc in ABAQUS ??

I am trying to model a disc in 2D as attached here. Because the disc is complete circular object, I tried to tie side A to B using MPC tie constraints. However, Abaqus gives several warnings for...

13 October 2020 2,040 11 View

How can I optimize qPCR result of miRNA from ticks?

Dear researchers, I am facing some issues with Sybre green qPCR results. I have isolated total RNA from different organs of ticks using the Trizol RNA isolation kit. After cDNA preparation with a...

14 September 2020 5,144 5 View

Thank's a lot again , I was reading about the reckittesiose and I know that the transmission is with a vector which is Tick? is Mosquito can transmit?

Is there any study published and demonstrated which indicates the presence of the agent of rickettsioses? in other arthropods not hematophage.

02 August 2020 5,506 3 View

How do I increase the space between maximum and minimum value in red marks on following graph (Note: graph is drawn in excel) ?

Hello friends, I am trying to increase the space (pitch) between the maximum and minimum values, which is indicated by red colour in the following graph. Although the difference between both...

31 July 2020 8,267 10 View

Fathy Ahmad Osman

- Once the tick has been removed from your body, however, there's nothing wrong with squashing it. It will kill it but you must put in your

Don't get their insides on you. An engorged tick is full of blood and other tick juices. If you are too squishing it, you're liable to get some on you..

Just make sure that you do it with a rock or boot instead of directly pinching it between your fingers. You should never handle a tick with bare hands. Their bodies are crawling with bacteria, so even if they're completely intact and not in any danger of regurgitating, they still aren't safe to handle.

Dharmesh C Patel

Tick may keep in a liquid nitrogen before squishing it so we can easily cruse with mortar and pestle

Alkhaled M. J.A

https://www.researchgate.net/deref/http%3A%2F%2Fwww.analytik-jena.de%2Fen%2Flife-science%2Fproducts%2Fprod%2Fcat%2Flysis-tubes%2Fprod%2Finnuspeed-lysis-tubes.html

David Burden

If you have a bead beater, try mixing small beads (100 micron) with a fly fishing bead that has a hole in it. Add an extraction buffer without detergent. It sounds crazy, but the large bead grinds the tick, forcing the parts through the hole like a mini French press. The smaller beads disrupt the bacteria.