Why very low percentage of reads are assigned to miRNA category by sRNAbench?

12 March 2020 1 426 Report

Hi,

I have miRNA Seq data. I am trying to predict miRNA from this data. However, I have seen almost half of the reads belong to 19-22bp (sequence length distribution) and another peak is detected at 32bp (I am assuming the possibility of piRNA). I am using sRNAbench for my analysis and I can find that more than 90% of reads are aligning into the reference genome. However, a very low percentage of reads are assigned for miRNA, details are the following:

Detected mature miR:2 (0.08%) Detected hairpin miR:2 (0.1%)Reads mapped to miRNAs:4 (0.0%)Reads mapped to miRBase hairpins:2 (0.0%).

Is it normal ? or I am missing something somewhere?

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