Could you please advise on the optimal method for dissolving recombinant proteins and any important precautions to consider during the process? I have purchased a lyophilized recombinant protein (IL-1 beta Protein, Mouse) from MedChemExpress, Cat. No. is HY-P7073. What is the recommended way to properly dissolve it? Should I add any specific buffer or stabilizers to maintain the protein’s activity and stability? Thanks a lot!
Reconstitute the protein in distilled water.
According to seller:
"Lyophilized from a 0.2 μm filtered solution of PBS or 50mM Tris-HCl, 50mM NaCl, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 6.8 or PBS, pH 6.8, 8% trehalose."
"It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose)."
https://www.medchemexpress.com/recombinant-proteins/il-1-beta-protein-mouse.html?srsltid=AfmBOoqFXaPwvnnyCJSNBeY4zctbfyfPbbw9R0TPwqI0LP6N3i6AD6rE
Wow! Really appreciate your help, Dr. Adam B. Shapiro! Seems like adding a carrier protein is super important.
Yes. Adam B Shapiro is right.
You may find the protein sticks to your plastic tubes, especially at low concentrations. That's why you will include a carrier protein. I recommend high purity BSA.
For lyophilized (freeze-dried) protein in powder form (example: 10 μg):
John Carter Hi, Is it okay to use either fatty acid-containing or fatty acid-free BSA?