How can I activate D-biotin for conjugation to a recombinant protein?

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Adam B Shapiro

https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/biotinylation.html

Omar Gonzalez-Ortega

It has to be activated. You could try to do your own activation (which I do not recommend, EDC chemistry sucks) using a carbodiimide with or without NHS.

John Carter

Use NHS-biotin, as Adam B Shapiro recommends.