1. Are there specific vectors available that help prevent false-positive results during cloning?
IN SENTENCE FORMATION
Please explain what your problem is and someone can perhaps make suggestions. But there are many vectors designed to aid in finding the right clone.
during cloning vectors i need
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
what all details we will get from fluorescence life time data
10 July 2024 505 1 View
I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....
24 June 2024 9,717 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
Dear colleagues, I am conducting a research study on the impact of performance-based incentives on project success in the IT industry. Your participation in this survey will provide valuable...
26 May 2024 3,890 3 View
There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...
14 May 2024 7,717 2 View
Hi, Planning to conduct a few case studies among diabetes participants. Is there any methodology available for the same?
05 May 2024 8,371 2 View
I have 24-hour temperature data for 30 days at 1-hour intervals. how could I compare and find days with similar climatic trends statistically?
17 April 2024 3,211 1 View
Purpose of Collaboration - 1. Collaborative Research in the field of nutrition, health, fitness 2. Knowledge Sharing in Nutrition domains 3. Writing articles in Indexed Journals If...
14 April 2024 7,563 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...
10 July 2024 5,966 2 View