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Questions related to Immunology Techniques
I have a polyclonal anti-MODV antibody as well as a monoclonal anti-flavivirus (E protein) antibody and would like to test both against MODV in cell culture. In developing a protocol I would like...
15 January 2017 6,014 4 View
Hi, I would like to study ROS production in GFP + cells. I tested DHE in order to detect superoxyde production, but I wanted a more global detection. I thought to CM-H2DCFDA but this probe is...
11 January 2017 5,632 3 View
Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...
04 January 2017 8,448 5 View
I would like to check the gene expression in the AS patients, but I don't know, I must measuring these genes in PBMC, macrophage or monocytes? Meanwhile, my mutant gene doesn't have any expression...
04 January 2017 8,632 2 View
Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...
19 December 2016 6,548 5 View
We try to detect macrophages and B-cells in rat bone-tissue. However, we get cross reaction when using mouse monoclonals. Has anybody experienced similar problems and developed a solution?
16 December 2016 3,053 4 View
Hi all, we would like to perform immune histochemistry to stain antigen-presenting cells on porcine lymph nodes. I found that SLA II DR and DQ are options, but which one would be best? Are there...
13 December 2016 9,714 3 View
Looking for feedback, positive or negative, from anyone who has used the LSBio.com bovine prolactin ELISA...
28 November 2016 6,891 0 View
My negative control (only cells non stimulated stained with FLICA) is stained as my positive control (LPS+MSU cystals). Does anyone have any idea what should I do in order to avoid it? The...
21 November 2016 5,710 4 View
I have been using Dynabead protein-G for immunoprecipitation of my target protein in a mix of antigen from two different protein samples. My target protein X is supposed to bind to the protein Y...
21 November 2016 6,691 4 View
For different donors, we observed the upregulated expression of immune checkpoint ligands on monocytes when they were cultured with only CD8 T cells (both antigen-specific and non-specific CD8 T...
20 November 2016 1,984 3 View
I will be using ELISA for detection of protein.
17 November 2016 8,849 4 View
I am particulary looking for anti somatostatin receptor antibody working in neural cortex of the mouse. If anyone have experience with good antibody for this field?
14 November 2016 956 3 View
We suspect that the 'ferocious' immune response that we observe is due to the presence of a conjugated ligand.
14 November 2016 1,915 3 View
I am going to use Immune complex for testing my antibody binding to FcgRIII on NK cells. The immune complex consists of the whole virus. But I want to get rid of unbound antibodies from IC...
08 November 2016 5,238 3 View
I need to test the specificity of a polyclonal antibody for use in western blot through a peptide competitive assay protocol.
07 November 2016 2,925 3 View
I need to add IFNg to my cell cultures and neutralize it (to determine if the mechanism of my findings are IFNg-dependent). Unfortunately there is no bovine anti-IFNg specific for neutralization....
02 November 2016 688 2 View
I would like to culture primary human monocytes, but without the cells attaching to the flask/plate. I´ve read in some papers the use of "teflon flasks" and "teflon plates", but have not been able...
21 October 2016 4,404 4 View
I need to measure IL-6 and TNF-α in human plasma samples. I need high sensitivity kits however ELISA kits are too expensive. Does anyone know if there is a way to increase sensitivity in the...
20 October 2016 8,785 3 View
Hi there, Any help on this possibly easily solved query will be gratefully received! I'm looking to analyse FCS files on FlowJo - the dot plot files display correctly but when I change the axis to...
13 October 2016 2,640 7 View
Hello everyone, I'm trying to use PI staining to determine cell cycle with flow cytometer. I use PI/RNase Staining Buffer (BD, 550825) and follow their protocal...
13 October 2016 2,530 3 View
Could someone share experience on the assay of Rat MCP-1 (Monocyte chemoattractant protein 1, also known as Chemokine Ligand 2 – a cytokine) in plasma/serum of rats using the RayBio MCP-1 ELISA...
09 October 2016 4,823 2 View
Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...
06 October 2016 4,924 3 View
Hi everyone, I am performing stereotactic surgery for mPFC in CD1 mice. The coordinates that I am trying to use is AP: +1.9, ML: +/- 0.3, DV: -2.5. Apart from this antero-posterior coordinates, I...
03 October 2016 244 5 View