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Questions related to Immunology Techniques
Does anybody know a better way to separate areas in a slide for immunofluorescence assays than the Super Pap Pen by Life Technologies? Because this pen is quite dirty, easy to wash away and not as...
16 January 2017 8,070 5 View
I had practiced mouse hepatocyte isolation with percoll for several month. The primary hepatocyte cells can only survive one day or less, and the cause of cell death is DNA released....
16 January 2017 418 0 View
I have a polyclonal anti-MODV antibody as well as a monoclonal anti-flavivirus (E protein) antibody and would like to test both against MODV in cell culture. In developing a protocol I would like...
15 January 2017 6,110 4 View
Hi, I would like to study ROS production in GFP + cells. I tested DHE in order to detect superoxyde production, but I wanted a more global detection. I thought to CM-H2DCFDA but this probe is...
11 January 2017 5,777 3 View
Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...
04 January 2017 8,593 5 View
I would like to check the gene expression in the AS patients, but I don't know, I must measuring these genes in PBMC, macrophage or monocytes? Meanwhile, my mutant gene doesn't have any expression...
04 January 2017 8,741 2 View
Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...
19 December 2016 6,659 5 View
We try to detect macrophages and B-cells in rat bone-tissue. However, we get cross reaction when using mouse monoclonals. Has anybody experienced similar problems and developed a solution?
16 December 2016 3,249 4 View
Hi all, we would like to perform immune histochemistry to stain antigen-presenting cells on porcine lymph nodes. I found that SLA II DR and DQ are options, but which one would be best? Are there...
13 December 2016 9,820 3 View
Looking for feedback, positive or negative, from anyone who has used the LSBio.com bovine prolactin ELISA...
28 November 2016 6,973 0 View
My negative control (only cells non stimulated stained with FLICA) is stained as my positive control (LPS+MSU cystals). Does anyone have any idea what should I do in order to avoid it? The...
21 November 2016 5,829 4 View
I have been using Dynabead protein-G for immunoprecipitation of my target protein in a mix of antigen from two different protein samples. My target protein X is supposed to bind to the protein Y...
21 November 2016 6,808 4 View
For different donors, we observed the upregulated expression of immune checkpoint ligands on monocytes when they were cultured with only CD8 T cells (both antigen-specific and non-specific CD8 T...
20 November 2016 2,098 3 View
I will be using ELISA for detection of protein.
17 November 2016 8,941 4 View
I am particulary looking for anti somatostatin receptor antibody working in neural cortex of the mouse. If anyone have experience with good antibody for this field?
14 November 2016 1,035 3 View
We suspect that the 'ferocious' immune response that we observe is due to the presence of a conjugated ligand.
14 November 2016 2,038 3 View
I am going to use Immune complex for testing my antibody binding to FcgRIII on NK cells. The immune complex consists of the whole virus. But I want to get rid of unbound antibodies from IC...
08 November 2016 5,329 3 View
I need to test the specificity of a polyclonal antibody for use in western blot through a peptide competitive assay protocol.
07 November 2016 3,048 3 View
I need to add IFNg to my cell cultures and neutralize it (to determine if the mechanism of my findings are IFNg-dependent). Unfortunately there is no bovine anti-IFNg specific for neutralization....
02 November 2016 776 2 View
I would like to culture primary human monocytes, but without the cells attaching to the flask/plate. I´ve read in some papers the use of "teflon flasks" and "teflon plates", but have not been able...
21 October 2016 4,503 4 View
I need to measure IL-6 and TNF-α in human plasma samples. I need high sensitivity kits however ELISA kits are too expensive. Does anyone know if there is a way to increase sensitivity in the...
20 October 2016 8,932 3 View
Hi there, Any help on this possibly easily solved query will be gratefully received! I'm looking to analyse FCS files on FlowJo - the dot plot files display correctly but when I change the axis to...
13 October 2016 2,746 7 View
Hello everyone, I'm trying to use PI staining to determine cell cycle with flow cytometer. I use PI/RNase Staining Buffer (BD, 550825) and follow their protocal...
13 October 2016 2,644 3 View
Could someone share experience on the assay of Rat MCP-1 (Monocyte chemoattractant protein 1, also known as Chemokine Ligand 2 – a cytokine) in plasma/serum of rats using the RayBio MCP-1 ELISA...
09 October 2016 4,921 2 View