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Questions related to Immunology Techniques
Which is better EDTA or heparinized tubes for mouse blood, I tried EDTA but it causes clotting sometimes although I put 1-2 ml blood only in the tube. And I tried heparin but it caused hemolysis ....
05 May 2015 915 5 View
Hello all, I am trying to induce NET induction by IL-8 (100 ng/ml) using healthy neutrophils and patients' neutrophils. I quantify the NET induction by Picogreen assay. In case of healthy...
05 May 2015 8,626 4 View
How does Brucella Abortus Strain 19 provide life long immunity in cattle in single vaccination, as it persist in mouse spleen for almost 4 months?
05 May 2015 4,614 2 View
I want to isolate monocytes and DC from liver. The protocol for density gradient cell separation I have now mentioned the speed for mononuclear cells (2500rmp, 20mins and all stuff should be room...
05 May 2015 7,500 3 View
I have tried WB specific markers, but I would like to somehow image my protein markers while imaging. Is there a pen that would help ? Or are there other innovative ways?
05 May 2015 4,779 3 View
We are planing implant decellularized xenograft subcutaneous to stablish the inflammatory answer we follow the implant for 1,2,7 and 15 days in CD-1 mice and we do immunosatining of the implanted...
05 May 2015 10,124 4 View
Gi often get nice pellets forming but they sometimes don't seem to run when I tilt the plate, should I count them as antibody positive.for example streaming ofpellets has occurred up until the...
05 May 2015 7,415 3 View
We have with us unconjugated primary antibodies that are compatible with flow cytometry and western blot. Since we are using a panel of antibodies We would like to label these for flow instead of...
05 May 2015 3,829 4 View
We are working with monocyte derived dendritic cells for immunotherapy purposes. As a part of cell culture control we want to test the cells for Endotoxine. We start using the LAL PYROGENT GEL...
05 May 2015 9,300 1 View
I am using C11-BODIPY 581/591 to stain B-progenitors (B220+ cells) from Black6 mice. I use flow cytometry to get a read out of the dye. The Life Technologies website mentions that to quantify the...
05 May 2015 415 1 View
We are looking for a reagent that reacts with native IgM pentamers carrying the J chain.
05 May 2015 7,788 0 View
Pl provide me cat log number of working anti-body for localizing LUBRICIN (PRG-4) in HUMAN TISSUE or in Human and Rat tissues both for Immunohistochemistry. Many thanks,
05 May 2015 7,980 5 View
I would like to determine the mechanism of diabetes development from one of our monkeys who had developed it as a result of treatment. We have hypothesized it being antibody mediated; does anyone...
05 May 2015 2,249 1 View
I work on Troponin I, I use 19C7 as a capture, and M18 for detection both at 1ug/mL, I need the assay to detect down to 0.1 ng/mL, and I'm just getting signal at 50 ng/mL. I use 2% BSA for block....
04 April 2015 9,654 3 View
how do you determine the appropriate cutoff for cytokine elisa to differentiate between positive and false positive?
04 April 2015 5,065 6 View
We would like to determine by flow all the cell types in the thymus that present OVA peptide on MHC II in RIPmOVA mice to OT-II T cells. There is an antibody towards OVA:MHCI complexes, but cant...
04 April 2015 2,513 2 View
Does anybody has experience with Competitive ELISA for PGE2? Should I perform the purification procedure of PGE2 from all samples? Thank you!
04 April 2015 5,446 7 View
Published literature shows that there are different types of methods used to determine the IgE binding of food allergens. After Skin Prick Test and Oral Food Challenge, What would be the best...
04 April 2015 3,651 4 View
We have a hypothesis that one of our cancer treatment method can induce specific antitumor immune response. I would like to measure it quantitatively in a simple Colon26 cell line derived...
04 April 2015 1,664 3 View
When a new drug is administered for its possible immune modulatory effect, how do we check the status of the immune system or the efficacy of the drug apart from the clinical signs.
04 April 2015 3,995 6 View
Will use porcine serum, to block in IHC both to reduce background and to save resources.
04 April 2015 3,918 3 View
I have differentiated THP1 monocytes into macrophage using 5 ng/ml PMA for 48 hrs. Then after washing twice with DPBS i have kept the diffrerentiated cells for 3 hrs in serum free media. Then I...
29 March 2015 6,908 8 View
Is there a method to induce the proliferation of dendritic cells or CD14+ T cells
03 March 2015 1,750 7 View
03 March 2015 1,575 8 View