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Questions related to Immunology Techniques
I have used anti-Calnexin antibody (1:200) from Santia Cruz (SC-23954) to do WB with human PTECs. I did not get the 90kDa band, but I did get the sharp band between 70-50 kDa and very weak band...
02 February 2016 2,555 2 View
I develop a lateral flow assay using PS microspheres conjugate. I realise my tests strips by strips and the assembly is handmade. I pretreat the sample pad with PBS Tw20 Sucrose buffer and dry it...
02 February 2016 9,315 1 View
The main problem: The tissue samples were obtained from rabbits. Beside, the primary antibody's host animal is rabbit. The third disaster: Our ABC staining kit is a universal kit. I have tried...
02 February 2016 3,078 6 View
I want to tag anti human antibody to target specific antigen invivo. the animal model will be mice. so I want to know whether this anti-human (higher species) antibody will create any toxocity to...
02 February 2016 3,708 0 View
I would like to test levels of heme in lymphoblasts. I see there are heme assays and hemin assays. What is the difference between the two?
02 February 2016 2,407 8 View
For BRCA1 and BRCA2 mutation , how can I use WB to detect them in tissue? Is there any effective antibody for doing this experiment? Thanks!
02 February 2016 1,841 0 View
Dear all, I'm going to quantify memory B cells using ELISPOT assay. I will use splenocytes from immunized C57BL/6 mice. Could you recommend any possitive control for this? Thank you very much!
02 February 2016 9,214 4 View
Hello i hope you are very well. i am kindly asking you. where is the best site to gating the osteoclast by Flow cytometry ? which is the suitable voltage to set up the forward scanter and side...
02 February 2016 8,288 2 View
Hello everybody, we are looking for a new flow cytometer, and I was wondering if any one has used the Miltenyi MACSquant flow cytometer. I am only used to BD machines. I would be particularly...
02 February 2016 4,177 2 View
Read 2 answers by scientists to the question asked by Abbas Ali Amini on Feb 1, 2016
01 February 2016 743 2 View
We are using KLH as an immunostimulant. I am trying to find the right filter size (guessing 20-40 micron range) for filtering the suspension before injecting in vivo to assess immune stimulation....
23 January 2016 6,819 3 View
can some one please tell me specific markers for Cardiac Fibroblast to confirm the presence of Cardiac Fibroblast?
22 January 2016 4,666 1 View
Hi every one, I transplanted bone marrow (BM) cells isolated from wild-type mice (C57BL/6 mice) into A gene knock-out mice (C57BL/6 mice) and vice versa. The irradiated mice without being BM...
08 January 2016 7,098 2 View
Actually, Cy7 NHS ester can be used in staining peptides. I want to stain the peptides that can bind to monocytes. Hence, I used Cy7 NHS ester for labeling the peptides and DAPI for labeling...
04 January 2016 2,204 1 View
01 January 2016 6,018 2 View
01 January 2016 4,592 1 View
Is it tricky to use MS to sequence a small recombinant portion of salmon IgT?
01 January 2016 2,299 0 View
01 January 2016 6,716 1 View
I grow cells in 8-well plates, then use receptor primary antibodies. This time I used a secondary antibody that we had, which was rhodamine conjugated. One of the wells showed a bright yellow...
01 January 2016 9,306 1 View
Kindly suggest me optimized PMA concentration that will work well for differentiating human monocytes THP1 into macrophages. I am facing problem with PMA concentration which is not helping THP1...
01 January 2016 1,987 3 View
01 January 2016 4,350 3 View
At the moment, the staining of the milk (immunoglobulines?) and the E. coli is too strong to evaluate the epithelial cells.
01 January 2016 8,369 12 View
I want to stimulate pDCs with immune complexes so i want them to be consisted of DNA-Antibodies
01 January 2016 4,512 0 View
Can anyone recommend any tool to determine the antigenicity, immunogenicity of a protein sequences?
01 January 2016 2,883 14 View