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Questions related to Immunology Techniques
The main problem: The tissue samples were obtained from rabbits. Beside, the primary antibody's host animal is rabbit. The third disaster: Our ABC staining kit is a universal kit. I have tried...
02 February 2016 2,959 6 View
I want to tag anti human antibody to target specific antigen invivo. the animal model will be mice. so I want to know whether this anti-human (higher species) antibody will create any toxocity to...
02 February 2016 3,625 0 View
I would like to test levels of heme in lymphoblasts. I see there are heme assays and hemin assays. What is the difference between the two?
02 February 2016 2,302 8 View
For BRCA1 and BRCA2 mutation , how can I use WB to detect them in tissue? Is there any effective antibody for doing this experiment? Thanks!
02 February 2016 1,753 0 View
Dear all, I'm going to quantify memory B cells using ELISPOT assay. I will use splenocytes from immunized C57BL/6 mice. Could you recommend any possitive control for this? Thank you very much!
02 February 2016 9,085 4 View
Hello i hope you are very well. i am kindly asking you. where is the best site to gating the osteoclast by Flow cytometry ? which is the suitable voltage to set up the forward scanter and side...
02 February 2016 8,180 2 View
Hello everybody, we are looking for a new flow cytometer, and I was wondering if any one has used the Miltenyi MACSquant flow cytometer. I am only used to BD machines. I would be particularly...
02 February 2016 4,070 2 View
Read 2 answers by scientists to the question asked by Abbas Ali Amini on Feb 1, 2016
01 February 2016 640 2 View
We are using KLH as an immunostimulant. I am trying to find the right filter size (guessing 20-40 micron range) for filtering the suspension before injecting in vivo to assess immune stimulation....
23 January 2016 6,729 3 View
can some one please tell me specific markers for Cardiac Fibroblast to confirm the presence of Cardiac Fibroblast?
22 January 2016 4,550 1 View
Hi every one, I transplanted bone marrow (BM) cells isolated from wild-type mice (C57BL/6 mice) into A gene knock-out mice (C57BL/6 mice) and vice versa. The irradiated mice without being BM...
08 January 2016 7,016 2 View
Actually, Cy7 NHS ester can be used in staining peptides. I want to stain the peptides that can bind to monocytes. Hence, I used Cy7 NHS ester for labeling the peptides and DAPI for labeling...
04 January 2016 2,108 1 View
01 January 2016 5,911 2 View
01 January 2016 4,493 1 View
Is it tricky to use MS to sequence a small recombinant portion of salmon IgT?
01 January 2016 2,183 0 View
01 January 2016 6,627 1 View
I grow cells in 8-well plates, then use receptor primary antibodies. This time I used a secondary antibody that we had, which was rhodamine conjugated. One of the wells showed a bright yellow...
01 January 2016 9,226 1 View
Kindly suggest me optimized PMA concentration that will work well for differentiating human monocytes THP1 into macrophages. I am facing problem with PMA concentration which is not helping THP1...
01 January 2016 1,855 3 View
01 January 2016 4,261 3 View
At the moment, the staining of the milk (immunoglobulines?) and the E. coli is too strong to evaluate the epithelial cells.
01 January 2016 8,264 12 View
I want to stimulate pDCs with immune complexes so i want them to be consisted of DNA-Antibodies
01 January 2016 4,436 0 View
Can anyone recommend any tool to determine the antigenicity, immunogenicity of a protein sequences?
01 January 2016 2,772 14 View
During a long passage history viruses may change their pathogenity and/or virulence for experimental animals and/or man. This phenomenon has a complex background. First, mutations may occur in...
01 January 2016 8,679 16 View
I use ready to use commercially available ELISA kit employing TMB/HRP method for detection of antigen. in TMB redox event what is supporting electrolyte?
01 January 2016 4,836 2 View