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Questions related to Immunology Techniques
Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...
06 October 2016 4,973 3 View
Hi everyone, I am performing stereotactic surgery for mPFC in CD1 mice. The coordinates that I am trying to use is AP: +1.9, ML: +/- 0.3, DV: -2.5. Apart from this antero-posterior coordinates, I...
03 October 2016 312 5 View
I am planning to perform T cell profiling of viral diseased samples of different progression patterns. Currently I am planning to check intra-cellular and phenotypic expression patterns, T cell...
30 September 2016 4,360 2 View
Does anyone knows how many NK cells per mice spleen we can get using the NK isolationKit II from Miltenyi Biotech?
29 September 2016 2,598 2 View
I want to use FACS sorter to sort/isolate monocytes from human BPMCs and based on literature CD14 is the best marker. After sorting Monocytes, I need the CD14 to be available/free for LPS and...
28 September 2016 7,831 2 View
The standard curves for all of the trials for this experiment have had R^2 values of above 0.9. I need to determine whether this is acceptable or whether I need to troubleshoot and improve my...
22 September 2016 3,771 2 View
Read 5 answers by scientists to the question asked by Aner Ottolenghi on Sep 20, 2016
20 September 2016 265 4 View
The samples may be required to be stored for a few weeks prior isolation.
20 September 2016 9,067 3 View
I try to purify virus-like particle that express in recombinant yeast. By sucrose cushion, then subject to equlibirum density CsCl centrifugation. After I culture yeast for 100mL, harvest, break...
21 April 2016 1,237 3 View
In some models of macrophage activation it can be triggered by the binding of immune complexes to Fc receptors. But in reality free antibodies can also bind FcRs (that's why we use the Fc block in...
19 April 2016 5,515 2 View
Hi, Some of the samples i processed have a red layer beneath the buffy coat and compromise greatly my cell count. Can it be safe to add a red blood cell lysing step to my PBMC isolation? Has...
12 April 2016 814 4 View
I am studying effect of DPSCs on T proliferation using PBMCs. The method I want to adopt used PHA as mitogen to activate PBMCS for two way MLR but for one way they have not clearly mentioned...
12 April 2016 9,089 3 View
I am currently working on optimizing a rapid (1.5 hr) stain for frozen human brain tissue using DAB. My positive slides would show staining, but so would my negative slides. I tried optimizing my...
11 April 2016 7,378 6 View
We use an easy protocol for platelets where we just spin out the RBCs then pellet the cells left in the Buffy Coat and it works well, I am wondering if the same can be done for Lymphocytes. Thanks
04 April 2016 10,028 3 View
molds have been cultured in PDA and incubated for over 28 days for possible OTA production. which OTA ELISA kit can best suit this kind of cultures
04 April 2016 2,334 2 View
04 April 2016 8,984 3 View
04 April 2016 8,363 5 View
04 April 2016 9,179 7 View
I'm looking for reliable zebrafish ago2 (eif2c2) antibody for IP. I found one form abcam (http://www.abcam.com/ago2--eif2c2-antibody-chip-grade-ab57113.html) (any experiences?) but its value/price...
04 April 2016 3,862 0 View
I am doing standard sandwich ELISA experiments and would like to know till what value of OD should be acceptable for the negative controls usually? I have used 7 rows for positive capture...
04 April 2016 4,039 6 View
I was using a metallic probe to have access to the trachea and I inject 500µl of PBS (Phosphate-buffered saline). Using the Biorad Milliplex Kit to quantify the cytokines in BAL, I have almost...
04 April 2016 5,873 2 View
If there is any ELISA kit for GD2 to order please let me know that or if there is not would you please help me with the protocol to do GD2 ELISA?
04 April 2016 848 1 View
I have a problem with my ELISA test. I bought biotinylated VEGF from R%D Systems and there is no signal after incubation with ABTS solution followed by incubation with strep-HRP conjugate. Before...
04 April 2016 6,511 3 View
I am interested in developing a monoclonal antibody against the human blood group A. Can anyone help to obtaining Glycoprotein fraction isolated from the saliva of secretors with blood group A,...
03 March 2016 4,541 4 View