I need to add IFNg to my cell cultures and neutralize it (to determine if the mechanism of my findings are IFNg-dependent).  Unfortunately there is no bovine anti-IFNg specific for neutralization. I found a recombinant bovine IFNg (143 aa, grown in yeast), and I found a bovine anti-IFNg against a 143 aa recombinant IFNg grown in yeast, but it is specific for ELISA and WB. Would that work to neutralize?

Someone from technical support recommended me to buy a human anti-IFNg (much much more expensive), but it doesn't really make sense to me... SOS!

If the anti-IFNg I found works to neutralize, can I confirm the neutralization with ELISA (using the rBoIFNg + anti-IFNg after incubation)?

Thanks!!