For different donors, we observed the upregulated expression of immune checkpoint ligands on monocytes when they were cultured with only CD8 T cells (both antigen-specific and non-specific CD8 T cells).
Expression was not observed when monocytes were cultured (without T cells) either alone or with a cancer cell line. Moreover, expression was not observed for 3-cell culture of monocytes, cancer cell line and non-specific T cells, but greatly upregulated when the 3-cell culture instead involved antigen-specific T cells.
(Media used contains 100IU/mL of IL-2 to 'keep T cells happy'.) Appreciate any input on this strange phenomenon.
Interferon gamma, which is secreted by activated CD8 T-cells on antigen-recognition, is known to induce PD-L1 expression. If PD-L1 was one of the checkpoint ligands you were monitoring, IFNg expression would explain this.
Interferon gamma, which is secreted by activated CD8 T-cells on antigen-recognition, is known to induce PD-L1 expression. If PD-L1 was one of the checkpoint ligands you were monitoring, IFNg expression would explain this.
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