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Questions related to Immunology Techniques
I want to measure the effect of Nanoparticles on macrophage metabolism, but I'm confused about the control. How can I be sure the result I'm getting is due to the effect of nanoparticles on cells...
03 March 2016 1,798 1 View
NURR1: Nuclear Receptor Related protein 1; MW = 67 kDa Hi Folks, I have been trying to detect overexpressed NURR1 in cell lysates but nothing has worked out yet. Following are the antibodies which...
03 March 2016 5,971 5 View
HI Dear Friends, I want to know that, does everyone know that how can I evaluate IgG and A by the methods of "Indirect Sperm Mar Test with Sperm Mar Kit"? On the other hand, can i use of human's...
03 March 2016 1,548 2 View
I would like to plate whole blood taken from mice via cardiac puncture for CFU counts. Does anyone have a protocol for this, specifically that addresses the use of heparin? I want to save most of...
03 March 2016 1,058 2 View
I took 200microL of serum sample, and isolated the RNA on the same day on which I isolated serum from the blood. The blood was also taken on the same day. TRIzol was used for the procedure, along...
03 March 2016 8,678 3 View
I am looking to measure the concentration of antigen-specific IgE antibodies in B6 mice (immunized with an antigen) using an ELISA, but I don't know if that will be possible. I know that the...
03 March 2016 2,445 2 View
Hello, I use LDH and/or Chromium release to evaluate NK cell cytotoxicity. I recently started to use ToPro-3 and/or AnnexinV to analyse killing by flow cytometry. The problem is that the % lysis...
03 March 2016 4,240 3 View
In my first experience, I cultured the PBMCs and isolate the adherent cells to the flask after 10 hours. After about 2 days the morphology of cells changed from a round small shaped-cells to the...
03 March 2016 8,185 4 View
Hi, Can peristaltic pumping system in cytoflex of BC show some adverse affect on the cell condition? As cells may need to pass through a narrow tube for peristaltic movement by the pump. Thanks
03 March 2016 10,146 1 View
I have been doing a lot of immunohistochemistry (IHC) experiments in an effort to get a certain antibody to work. I look for co staining with other cells in the spinal cord. I used tyramide...
03 March 2016 8,300 7 View
Hi, I plan to use CD14+ monocyte from human PBMC to get polarized Macrophage. I need large amount of Macrophages (around 5x10^8). However, I can only get limited blood samples. So I would like to...
03 March 2016 514 9 View
I am curious to know if anyone has experience conducting B-cell fusions for hybridomas from Peyer's patches? If so, what species/strain, is there variability dependent on antigen, what...
03 March 2016 1,901 1 View
I'm planning to investigate a hormone human Chorionic Gonadotropin(hCG) by using immunoassay method on glass slides. I'm really looking to find out how to analyze these immuno-complex.
03 March 2016 7,413 0 View
I have established a new zebrafish lab. At present I face with the problem of fish mating and reproduction. Though the female fishes are mature enough and receive enough food they are not laying...
25 February 2016 5,797 2 View
I have used anti-Calnexin antibody (1:200) from Santia Cruz (SC-23954) to do WB with human PTECs. I did not get the 90kDa band, but I did get the sharp band between 70-50 kDa and very weak band...
24 February 2016 7,547 2 View
The main problem: The tissue samples were obtained from rabbits. Beside, the primary antibody's host animal is rabbit. The third disaster: Our ABC staining kit is a universal kit. I have tried...
23 February 2016 9,674 5 View
Dear all, I'm going to quantify memory B cells using ELISPOT assay. I will use splenocytes from immunized C57BL/6 mice. Could you recommend any possitive control for this? Thank you very much!
16 February 2016 9,521 3 View
I would like to test levels of heme in lymphoblasts. I see there are heme assays and hemin assays. What is the difference between the two?
10 February 2016 2,512 7 View
Following CD4 T cell depletion, how long should i hang the anti-CD4 in the blood? If i want to do adoptive transfer of CD4 T cell, how long should I wait to have the least effect due to...
02 February 2016 730 2 View
Read 2 answers by scientists to the question asked by Abbas Ali Amini on Feb 1, 2016
02 February 2016 4,964 2 View
I am confused...can pro inflammatory act as an anti-inflammatory or inflammatory cytokines. Literally "pro" means "before" and "anti" means "against". So my thinking is pro-inflammatory is...
02 February 2016 1,104 4 View
I'm trying to detect human CD63 by western in cell lysates and exosome fractions. I've had good results in the past with Santa Cruz H-193, but not so much anymore.
02 February 2016 3,728 2 View
I am unsure of the potenital cytokine profiles after the stimulation of splenic cells after immunization with different adjuvants (different mice). Since the adjuvant influences the cytokine...
02 February 2016 4,550 3 View
I am trying FISH on mouse brain tissue, but I got cross reactivity between the miR signal and the antibody I used. Brains are fixed with 4% PFA, cryopreserved and sectioned (12.5um thickness). I...
02 February 2016 6,847 2 View