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Questions related to Immunology Techniques
Hi, I would like to use Vybrant phagocytosis assay, using fluorescently labeled e.coli Bioparticles, to measure phagocytosis in BM derived neutrophils. I would like to read the samples on the...
16 December 2015 9,595 7 View
Hi. I am working on Experimental autoimmune encephalomyelitis (EAE). I followed a general protocol, 9-12 weeks old female C57BL/6 mouse was immunized with emulsion (emulsified in 1:1 volume ratio)...
14 December 2015 3,573 44 View
I used Sigma antibody raised in goat for WB against IL-1 beta using human CSF samples.
12 December 2015 7,571 6 View
I am trying to do some experiments on peripheral blood lymphocytes. And for that purpose I need to keep these primary cells alive in culture medium for a day or two. Does anybody know if they will...
12 December 2015 1,600 7 View
12 December 2015 4,113 8 View
I'm trying to reconstitute the immune system of irradiated Balb/c mice with LSK sorted cells, but they die every time. I tried to decrease the irradiation doses, they die later, but they still...
12 December 2015 770 3 View
We are testing the anti PD-1(Ketruda) activity on human PBMCS in vitro. Is there any recommended concentrations that are being used? I would be grateful for some references in that...
12 December 2015 3,632 5 View
I am looking for anti-LuxAB antiserum. If someone could help us in getting small amounts of these antibodies?
12 December 2015 5,682 0 View
I'm going to study the anti-inflammatory effects of carvacrol and thymol in vitro using ELISA kits for TNF-a and IL-6. I have to use only monocytes? I'm going to use cells from patients with...
12 December 2015 3,245 13 View
Medical student investigating effects of DPP-4 inhibitors in piriform cortex (olfaction) in diabetic GK rat brain. Primary antibodies: mouse anti-NeuN (1:300), and rabbit anti-Calbindin-D28k...
12 December 2015 7,047 5 View
01 December 2015 7,575 12 View
Can thioglycolate pass through 0.22 um filter for sterilization?
27 November 2015 5,814 2 View
I want to inject ovalbumin into zebrafish (i.p.) to result in immunoreaction, so how much OVA I need for one fish? In addition to OVA, do I still need to inject some LPS?
26 November 2015 2,473 1 View
Is it possible to use HRP-conjugated secondary Ab (for detection Ab in sandwich ELISA) without using the biotin-avidin? Please help me to solve it?
11 November 2015 8,384 3 View
I need a recipe for making protein free blocking buffer for ELISA and WB use
11 November 2015 3,928 10 View
Currently I am working with Acanthamoeba on the open bench, so I want to know if any containment conditions to work with this parasite.
11 November 2015 8,697 3 View
11 November 2015 4,319 2 View
I have always labeled cells with CFSE after detaching them from the culture plate. However I need to label plated macrophages this time. What I usually do is use the required concentration of CFSE...
11 November 2015 1,574 4 View
Hello everyone, I am wondering if anyone knows if there is anything against using a mouse primary antibody on mouse tissue for ChIP. I know that using same-species antibodies for IF/IHC isn't...
11 November 2015 2,853 8 View
I am using EDC/NHS/MES buffers but the antibody I want to conjugate is really expensive so I am trying to optimise the protocol using BSA. The protocol I have been using is to add 10mg of NPs with...
11 November 2015 2,460 2 View
I have read a paper in Nature on isolation of lpmc's from mouse colon tissue (2007, nb.10, page 2308). It is really interesting and I would like to give it a go. However, I am a bit concerned with...
11 November 2015 9,052 1 View
The cell culture supernatant of electroporated ADMSCs expressing VEGF has to be analyzed. The expressed VEGF (target for western blot) will be released in low glucose DMEM containing 10% FBS and...
11 November 2015 1,509 6 View
I want to titrate my rabbit anti-human FPR1 antibody and would like to perform the titration on cells that I know strongly express FPR1 on their surface.
30 October 2015 9,549 3 View
10 October 2015 1,764 4 View