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Questions related to Immunology Techniques
During a long passage history viruses may change their pathogenity and/or virulence for experimental animals and/or man. This phenomenon has a complex background. First, mutations may occur in...
01 January 2016 8,746 16 View
I use ready to use commercially available ELISA kit employing TMB/HRP method for detection of antigen. in TMB redox event what is supporting electrolyte?
01 January 2016 4,884 2 View
Hi, I would like to use Vybrant phagocytosis assay, using fluorescently labeled e.coli Bioparticles, to measure phagocytosis in BM derived neutrophils. I would like to read the samples on the...
16 December 2015 9,662 7 View
Hi. I am working on Experimental autoimmune encephalomyelitis (EAE). I followed a general protocol, 9-12 weeks old female C57BL/6 mouse was immunized with emulsion (emulsified in 1:1 volume ratio)...
14 December 2015 3,636 44 View
I used Sigma antibody raised in goat for WB against IL-1 beta using human CSF samples.
12 December 2015 7,643 6 View
I am trying to do some experiments on peripheral blood lymphocytes. And for that purpose I need to keep these primary cells alive in culture medium for a day or two. Does anybody know if they will...
12 December 2015 1,677 7 View
12 December 2015 4,182 8 View
I'm trying to reconstitute the immune system of irradiated Balb/c mice with LSK sorted cells, but they die every time. I tried to decrease the irradiation doses, they die later, but they still...
12 December 2015 818 3 View
We are testing the anti PD-1(Ketruda) activity on human PBMCS in vitro. Is there any recommended concentrations that are being used? I would be grateful for some references in that...
12 December 2015 3,707 5 View
I am looking for anti-LuxAB antiserum. If someone could help us in getting small amounts of these antibodies?
12 December 2015 5,722 0 View
I'm going to study the anti-inflammatory effects of carvacrol and thymol in vitro using ELISA kits for TNF-a and IL-6. I have to use only monocytes? I'm going to use cells from patients with...
12 December 2015 3,321 13 View
Medical student investigating effects of DPP-4 inhibitors in piriform cortex (olfaction) in diabetic GK rat brain. Primary antibodies: mouse anti-NeuN (1:300), and rabbit anti-Calbindin-D28k...
12 December 2015 7,116 5 View
01 December 2015 7,665 12 View
Can thioglycolate pass through 0.22 um filter for sterilization?
27 November 2015 5,870 2 View
I want to inject ovalbumin into zebrafish (i.p.) to result in immunoreaction, so how much OVA I need for one fish? In addition to OVA, do I still need to inject some LPS?
26 November 2015 2,535 1 View
Is it possible to use HRP-conjugated secondary Ab (for detection Ab in sandwich ELISA) without using the biotin-avidin? Please help me to solve it?
11 November 2015 8,436 3 View
I need a recipe for making protein free blocking buffer for ELISA and WB use
11 November 2015 4,004 10 View
Currently I am working with Acanthamoeba on the open bench, so I want to know if any containment conditions to work with this parasite.
11 November 2015 8,783 3 View
11 November 2015 4,382 2 View
I have always labeled cells with CFSE after detaching them from the culture plate. However I need to label plated macrophages this time. What I usually do is use the required concentration of CFSE...
11 November 2015 1,638 4 View
Hello everyone, I am wondering if anyone knows if there is anything against using a mouse primary antibody on mouse tissue for ChIP. I know that using same-species antibodies for IF/IHC isn't...
11 November 2015 2,899 8 View
I am using EDC/NHS/MES buffers but the antibody I want to conjugate is really expensive so I am trying to optimise the protocol using BSA. The protocol I have been using is to add 10mg of NPs with...
11 November 2015 2,505 2 View
I have read a paper in Nature on isolation of lpmc's from mouse colon tissue (2007, nb.10, page 2308). It is really interesting and I would like to give it a go. However, I am a bit concerned with...
11 November 2015 9,123 1 View
The cell culture supernatant of electroporated ADMSCs expressing VEGF has to be analyzed. The expressed VEGF (target for western blot) will be released in low glucose DMEM containing 10% FBS and...
11 November 2015 1,563 6 View