I have a polyclonal anti-MODV antibody as well as a monoclonal anti-flavivirus (E protein) antibody and would like to test both against MODV in cell culture. In developing a protocol I would like to first test binding ability of these antibodies directly (not in cell culture), but unfortunately do not have purified antigen.
Is it possible to adsorb virus directly to a culture vessel while maintaining antigenic structure? Suggestions for other approaches would be greatly appreciated as well.
Hello
See links here will help you
Good Luck
https://www.aphis.usda.gov/animal_health/vet_biologics/publications/VIRPRO1014.pdf
https://www.agriculture.gov.ie/media/migration/animalhealthwelfare/veterinary/virology/tests/Testdescriptions.pdf
Thank you Saleh. However, I am wondering if it is possible to directly bind virus to the culture vessel, rather than fixing cells which have been previously infected. Like an ELISA, but with whole virus in place of purified antigen.
Yes, I've seen virus purification performed in sucrose gradient or using sequential filters. The purified virus could then either be used in a Western Blot or in an ELISA format. For the latter, you would need a secondary antibody that recognizes the Fc portion of the species that generated the primary Ab.
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