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Questions related to Immunology Techniques
I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...
03 March 2015 6,138 2 View
Hi, We've been trying to perform Ca2+ flux experiments by dying cells with FURA-2, and then measuring fluorescence using a plate reader in a 96-well format. The protocol has worked beautifully...
03 March 2015 6,959 3 View
It is antibiotic susceptibility test involving diluting wells . But I dont know how to carry out and what the resuls mean
03 March 2015 5,418 3 View
We have stimulated our PBMCs from normal controls with PMA (5-50 ng/ml) and Ionomycin/ 1ug/ml. Our target is to assess the functions of NK cells (both cytokine release and cytotoxicity) in these...
03 March 2015 1,995 11 View
We are studying NK cell functionality and observe a hyporesponsiveness of NK cells in a distinct setting. These NK cells have lower levels of IFN-g measured by ICS. We also observe that...
03 March 2015 571 0 View
The majority of experiments performing adoptive transfers of lymphocytes (T or B) into immunodeficient mice inject the cells into the recipient animal by intravenous (IV) means. This is usually...
03 March 2015 3,727 21 View
I am looking to mark this cellular subset for identification via flow cytometry of bone marrow samples. Any help would be appreciated.
03 March 2015 7,691 1 View
How can we separate young and old mouse neutrophils? What are these cell's significant characteristic? What kind of techniques do you use to separate these cells?
03 March 2015 6,143 5 View
I want to test IL10 secretion from B lymphocyte. Do I need to use Monensin or Brefeldin A for blocking? Kindly clarify whether or not Monensin is a B lymphocyte blocker and Brefeldin A is for T...
03 March 2015 8,885 6 View
I am trying to determine antibody concentration of immunized mice by incubating transfected HEK cells with mice sera. As I have many samples, it should be established in a 96 well plate. I detach...
04 February 2015 9,906 11 View
I would like to know if anyone has carried out ELISA assessment of TRPV1 using blood serum. I would also like to know if anyone has done western blotting of TRPV1 protein in whole brain...
02 February 2015 8,033 4 View
There's an interaction with a protein, FAP2, on a bacteria, F. Nucleatum, with TIGIT on natural killer cells. I want to target the natural killer cells specifically to block this interaction...
02 February 2015 6,374 2 View
i am coculturing t cells with other cell types and after co culture i want to isolate only t cells and eliminate other cells which this t cell is interacting so which method can i follow let it be...
02 February 2015 404 7 View
Should it be related to the protein size or any other parameters? The antigen size is aprox. 12 kDa.
02 February 2015 9,744 2 View
What is the statistical method that can be used to evaluate performance characteristics of immunoassay results in absence of gold standard rather than Bayesian theorem ?
02 February 2015 1,271 1 View
whether structural proteins are good for vaccine development or non-structural proteins?
01 January 2015 8,216 5 View
Hello all: I am interested in isolating pig monocytes from whole blood. I will do so by Ficoll followed by MACS for CD14 marker. My 2 questions are: does anyone know the best culture media for...
01 January 2015 8,593 4 View
I want to adoptive transfer some cells into recipients' thymus. But I have no experience on that. Can anyone who has some experience on this method tell me how to do this experiment?
01 January 2015 6,013 2 View
I prepared nitrated and aggregated protein.
01 January 2015 7,434 3 View
Recently I checked the acsf pH before and after 95% O2/ 5% CO2 buffering and found pH value is 7.8 (7.8) and 7.6 (after) with 26mM NaHCO3 and 5% CO2. Does anyone know how to calculate the buffer...
01 January 2015 9,167 5 View
I am trying to prepare an anisomycin solution for injection into the brains of my rats. I am dissolving the ANI in equimolar HCl ( 1 mmol of ANI for 1mmol of HCl). The ANI dissolves easily in HCl....
01 January 2015 6,254 2 View
Hello everybody, I have to induce M1 activation with IFN gamma and LPS on murine macrophages. Do you advise to proceed in serum or serum-free conditions? I have read different protocols about this...
01 January 2015 1,025 14 View
What is best method for obtaining Fetal hemoglobin from red blood cells? Do you think it is mass spectrometry? I am open for any suggestions.
01 January 2015 3,297 7 View
In the pregnancy test strip there are two types of antibodies monoclonal ab and polyclonal ab, the monoclonal ab is immobilized to the strip but when the sample touches it it gets detached and...
01 January 2015 1,452 3 View