Gene synthesis - pros and cons of codon optimization?

Dear all, I am synthesizing a nematode gene for reexpression in human cells and functional characterization (not for protein expression/purification). What are the pros and cons of codon...

04 November 2019 2,399 2 View

How to estimate regression to find which firm-specific characteristics explain cross-industry heterogeneity in the firm returns?

I would kindly like to ask for guidance regarding my question below. To illustrate my question: I have two industries: Industry 1 and Industry 2. Both industries contain a number of firms that...

20 May 2019 4,907 1 View

Is there a method to make a serum working on paraffin embedded tissue?

We have raised a rabbit antiserum against a protein coupled peptide. This peptide has been reported to be antigenic and some papers have been published with a commercial preparation raised in the...

22 January 2017 5,681 4 View

Can anyone recommend antibodies for histochemistry of rat macrophages and B-cells?

We try to detect macrophages and B-cells in rat bone-tissue. However, we get cross reaction when using mouse monoclonals. Has anybody experienced similar problems and developed a solution?

15 December 2016 2,972 4 View

Which exon should I target for CRISPR-Cas9 system?

Is there any rule/preference which exon should I target to knock out the GOI by CRISPR-Cas9 system?

13 November 2016 4,728 5 View

Does anybody know a simplified methodology or equation to estimate the temperature of a solar collector/absorber?

Does anybody know a simplified methodology or equation to estimate the temperature of a solar collector/absorber? I want to calculate the energy performance and the energy yield. I have Data for a...

17 March 2016 4,911 5 View

Does my ligated plasmid ready for sequencing and is the purity OK?

I performed a ligation of 1.5 kb insert into a 4.2 kb vector and I got colonies on my kanamycin plate. I picked up 8 colonies and made a miniprep of them. 2 out of the 8 samples contained the...

15 February 2016 860 3 View

How do we solve our problem with mouse hippocampal cell cultures on 35 mm petri dishes?

Hi there,We have some problems with (E17-18) mouse hippocampal cell cultures. After sacrificing mice (E17-18), primary neurons were cultured (400,000 cells per on the 35 mm petri dishes). For...

08 February 2015 7,460 6 View

Mammalian expression vector for isolation of native protein complexes?

Hi, Can anyone recommend a specific mammalian expression vector that I could use to tag my protein of interest and that would allow me to purify any associated complexes for MS analysis? I am...

13 July 2014 5,410 10 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene of interest?

Hi, I have my gene of interest in pcDNA5/FRT/TO and would like to generate a stable cell line. Before generating the stable line, however, I would like to test expression by transient...

06 July 2014 132 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

Safety of the SARS-Cov-2 mRNA vaccine?

Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...

04 February 2021 721 6 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Fuzzy regulator applied to the asynchronous machine?

hello, in fact I encountered problems during the simulation of the fuzzy regulator applied to the asynchronous machine. I replaced the PI regulator of the vector control with the fuzzy regulator,...

12 January 2021 6,131 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View