Are there any good specialists on the packages "leaps" and "SpaMM" in the R environment for collaboration?

My colleagues and I have done work on the polymorphism of flower color in one of the higher plant groups. But, unfortunately, it was rejected due to incorrect statistical analysis. We are just...

10 December 2020 885 3 View

Please help me identify this Diptera. This family Tephritidae?

Collected September 2, 2016 in Donetsk, Ukraine

10 September 2016 930 17 View

Is this DiI staining valid or specific?

I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...

01 February 2016 2,766 17 View

What is the most accurate and affordable pipette company?

27 October 2015 7,879 3 View

Can anybody recommend a good text on proposal writing and literature synthesis?

I am looking for suggestions from experts-what they suggest and students-what worked for you?

05 June 2014 3,721 3 View

Plant organ preservation in RNAlater for next generation transcriptome sequencing?

Does anyone have an RNA preservation protocol that yielded good next generation transcriptome sequencing results from preservation of plant organs in RNAlater (or another preservative) under field...

11 March 2014 1,359 3 View

How to make calculations with taking H2SO4 into account as a solvent in Gaussian 09?

I'd like to calculate relative energies for some molecules with taking a solvent into account. Reaction takes place in sulfuric acid, which is not included in the list of defined solvents in...

15 October 2013 7,150 7 View

How can one activate resting B cells and how can you check if they were really activated?

Dear colleagues, We are going to stimulate human B lymphocytes isolated from PBMCs from blood of healthy subjects (negative selection with MyltenilBiotech magnetic beads). It should be a control...

24 May 2012 2,800 29 View

KR20 for reliability of the survey instrument. Looking for clarifications?

I am working on my thesis and I have a survey that has true/false and multiple choice questions. From what I understand, KR20 is used for dichotomous variables to estimate reliability coefficient....

01 January 1970 789 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View