Immunohistochemistry of liver sections for oxidative stress?

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I would be glad if someone assists me to how analyze the results of mouse TNF Alpha ELISA Assay?

I have conducted a Mouse TNF alpha ELISA assay for protein determination in mice serum. I used byonoy Absorbance 96, to analyze the ELISA plate. I already prepared the standard curve and now, I...

20 May 2021 656 2 View

I would be glad if someone assists me to prepare standard curves for the determination of glutathione (GSH) and glutathione disulfide (GSSG)?

Dear Scientific Community, I would be glad if someone assists me to prepare standard curves for the determination of glutathione (GSH) and glutathione disulfide (GSSG). I need to determine GSH and...

11 April 2021 4,980 3 View

Does anyone have a protocol for doing platelet activating factor (PAF) from serum?

We did 12 patients and got very high values using a PAF ELISA and then when we did 8 more patients (with same disease severity) using the same PAF ELISA we got much lower values. 1. Is it OK to...

06 February 2014 4,771 1 View

How to do ICS for IL-10 in B cells?

All protocols mention that when you do ICS for IL-10 to identify regulatory B cells that before surface staining you have to block with antibodies for Fc receptors. Can anyone explain why please?

22 January 2014 6,696 3 View

Can anyone help with FSC and SSC using Partec 4 colour flow cytometer?

Has anyone had any problems with setting the FSC and SSC and getting rid of the background noise with a partec cube 4 colour flow cytometer? Also when we use Percep in the FL3 channel it gives a...

02 January 2014 9,119 1 View

Does someone have a protocol to quantify dengue viruses?

Any dengue virus would do, we don't necessarily want to know the serotype. We just want to measure the viral loads in serum in general.

21 November 2013 6,067 2 View

Expression of data as MFI or percentage of positive cells in flow cytometry?

If we are looking at a receptor expression on T cells (IL-10R) is it better to use the % of positive cells, mean fluorescence intensity, or median fluorescent intensity?

21 November 2013 3,103 5 View

Why do we get very hazy plaques in our plaque assay for dengue?

how can we get clearer plaques? we use BHK-21 cell lines.

27 January 2013 6,534 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

About impact factor?

I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...

01 August 2024 1,277 1 View

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (E-ISSN 1901-9726, P-ISSN : 0900-9671) a legitima?

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...

01 August 2024 629 3 View

Edson R. A. Oliveira

Try looking at PMID: 27788262. Its a recent paper with some interesting markers about what you need.

Saleh Alkarim

Hello

Also papers here will help you

Good Luck

https://www.ncbi.nlm.nih.gov/pubmed/21812977

https://www.ncbi.nlm.nih.gov/m/pubmed/22906265/

https://www.spandidos-publications.com/etm/1/5/809/download

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661801/

Gathsaurie Malavige

Thanks a lot for all these papers.