Which temperature is optimal for storage? Shall I fix slides before storage?
Few items to consider here before proceeding:
1. Fixation before freezing or cold storage of any kind is recommended. Freezing first can cause crystals and other things to damage the cells. Will fixation effect your downstream application? Some proteins are greatly effected by fixation. Fixatives used could include: Acetone, Methanol, Buffered Formalin, etc
2. The temperature recommended after fixation is -80C, unless your protein of interest is damaged at that temperature.
3. Just like anything biologic, long periods in a -80C freezer will dehydrate and desiccate your samples, I would not keep them in there very long
Great Suggestions found on IHC World Here: http://www.ihcworld.com/smf/index.php?topic=1398.0
Few items to consider here before proceeding:
1. Fixation before freezing or cold storage of any kind is recommended. Freezing first can cause crystals and other things to damage the cells. Will fixation effect your downstream application? Some proteins are greatly effected by fixation. Fixatives used could include: Acetone, Methanol, Buffered Formalin, etc
2. The temperature recommended after fixation is -80C, unless your protein of interest is damaged at that temperature.
3. Just like anything biologic, long periods in a -80C freezer will dehydrate and desiccate your samples, I would not keep them in there very long
Great Suggestions found on IHC World Here: http://www.ihcworld.com/smf/index.php?topic=1398.0
The cytospin slides for IHC can be stored theoretically for indefinite time for IHC.
The most important factor is FIXATION AND PREVENTING smears from getting dry.
For storage longer than an year , they can be fixed with methanol and kept at 4-8 degrees for as long as 2 to 3 years. It is our personal experience of storing the fixed slides in methanol for nearly 18 months and subsequent application of IHC on cytospin slides.
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