Hello, I need to look for expression of IDO on myeloid dendritic cells (CD1c, CD141) on bone marrow core biopsies. IDO is different from most antibodies in that the antigen retrieval solution is specifically proteinase K. As opposed to the antibodies for CD1c and CD141, which require a high or low pH antigen retrieval solution. We have no protocol to reference if this is even possible, mixing proteinase K with a low/high pH on the same slide. Any advice is greatly appreciated.
Ahmed R.H. Ahmed
Hello. You should consider the possibility that proteinase K (used for IDO) could affect the epitope of CD1c and CD141; and hence may affect their detection by the antibodies. In my opinion, it is better to perform antigen retrieval steps separately. Good luck.
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