Hi everyone!
We are working with corneal endothelium on Descemet membrane. It can be considered as a cell monolayer on thin protein membrane, so you can treat it like a fresh tissue slide. We want to perform IHC. In a paper where they studied the same tissue they have air dried fresh Descemet membrane on a slide, then "fixed in acetone and stored at -20C".
https://iovs.arvojournals.org/article.aspx?articleid=2600835
The question is how to store this tissue slides after acetone fixation at -20C - dry (wait until acetone evaporate and put a slide in some plastic bag and leave at -20C) or in acetone (leave slide in a bottle with acetone and store it at -20)?
Acetone play a role of precipitating sugars and fat as well as maintain the immunologic competence and is often used as it has a strong penetrability and dehydration property. So, leaving slides in acetone at -20C might over dehydrate.
Nadia, Bashir and Nirali, thanks a lot! Will keep slides dry and at room temperature
the slides can be filed in a cabinet under dry and room temperature.
Fresh tissue slides mean preferably frozen section tissue slide I consider. Thus for such slides, it is appropriate to store the acetone fixed slides in a sealed slide box without acetone at -80degrees for later use....
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