Hi!
I'm analyzing some TMA IHC cores using an scoring method that combines intensity and proportion of stained cells. I would like to know if is there any protocol to quantify percentage of DAB stained cells (the markers I'm using stains both cytoplasm and nucleus) and if is there any that can be automated (using ImageJ maybe?) since I have plenty cores?
I found this article Article Semi-quantitative Determination of Protein Expression Using ...
to quantify OD. That would be nice if there was something similar to percentage assigning.Thanks.
QuPath has an automatic TMA tool that can automatically detect and map the cores. It also has all the toolkits required for quantifying cells on whole slide level.
(https://qupath.github.io)
QuPath is what you should look for your purpose. Plus they have video tutorials on youtube as well so makes it easy
https://qupath.github.io/
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