What can I do to eliminate non-specific isotype control staining in immunohistochemistry?

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What is the difference betwenn 6-311G(d, p) basis set and 6-311g(2d, 2p) basis set ?

Gaussian

29 May 2023 9,916 4 View

How do I connect the Electrodes for external Quantum Efficiency (EQE) measurement?

Hello , I'm trying to take external quantum efficiency measurements. I'm measuring the p-type photocathode thin film in a NaCl liquid in a three-electrode system (pt, Ag/AgCl and working...

18 April 2023 2,259 0 View

How do I measure the concentration of miRNA?

We will study with SH-SY5Y cell lines in our project. We will isolate total RNA by using innuPREP RNA Mini Kit. We will use total RNA as starting material for cDNA synthesis. How can we measure...

05 July 2020 9,601 1 View

Any Journal recommendations for Covid-19 research article?

I'm working on a research article on the subject of 'Psychological and social impacts of Covid-19 pandemic in Turkey'. I'm looking for an SCI journal to publish my Covid-19 related article with no...

03 May 2020 2,319 11 View

Is ther any one working on The performance of Banks in Transient Countries?

For cooperative studies, I am looking for group members.

10 January 2015 5,466 8 View

Is there any significant differences between university spin-offs and innovative start-ups?

We live in a rapidly changing global competitive environment where new knowledge is created, disseminated, and commercialized to provide innovative products and services. In this global...

13 January 2014 2,880 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View