Hi,

The autofluorescence of the retinal pigment epithelium is a problem.

I have used 0.3% KMnO4 for bleaching the pigment epithelium layer on cryosections. Also, I have used Oxalic acid in a concentration below 1%. Incubation times varied from 5-15 minutes at RT. In both cases I performed the bleaching before the blocking step in IHC.

Be carefull with the sections on your slides because the bleaching treatment makes them even more vulnerable.

I would suggest you to just try out different conditions (concentrations, temperature and incubation time).

Goodluck!

thanks for answering my question Martin, which method worked better for you?

Hi,

I tried both seperately and in the end I combined them. So an incubation with one, followed with washing and an incubation with the other. This was succesfull. I would suggest you try this out. If your slides are too bad after treatment, decrease concentration and/or incubation time.

Goodluck!