How to do a meta analysis--defining a model?

Hi, I wanted to know the fundamental basics of a meta analysis. I have read up and found many diff methods being applied to answer a given research question. I want to understand the below: 1....

20 July 2020 4,445 5 View

Does the concentration of a sample affect the absorbance measurement in the visible region?

The two plots in the attached figures illustrate spectra for copper-polyethylenimine (Cu-PEI) and copper-polyacrylic acid (Cu-PA2) complexes. In the spectrum for Cu+1.7 ppm PEI (first plot) there...

10 July 2020 8,306 3 View

Has anyone used Python for qualitative research analysis?

I am looking into the benefits of using programming skills for data coding and analysis. This stems from the need for highly technical skills when considering roles outside academia. If you have...

23 June 2020 9,142 1 View

What are the most important features which a student appreciates in a teacher?

I would like to get some feedback from students on what are some of the things which they like in a teacher. Any suggestions or comments from regular university students would be highly...

19 April 2020 1,270 20 View

What are some of the best ways of publishing research work ?

I have done research and now would like to share or publish my research. What are some of the best ways to do it? Any suggestions or comments from expert researchers who have published many...

19 April 2020 7,146 8 View

How to become an expert in Data Science ?

I would like to know how can someone who is a beginner in the field of Data Science become an expert in this field within a short span of time. Any suggestions or comments from expert Data...

12 April 2020 5,075 10 View

Can online education replace traditional classroom education?

I wonder if we can completely replace the education system from physical classroom mode of teaching to an online based teaching system so that we can close down all the university buildings and...

09 April 2020 938 33 View

What are some tips for students during lockdown due to COVID-19?

I want some ideas from professors and researchers regarding how students can make best use of their time and focus on their studies during this nationwide lockdown period in different schools and...

07 April 2020 8,049 16 View

Why is India not yet a developed country?

I wonder when will India get fully developed as it is still developing for many years. Any suggestions or comments from researchers would be highly appreciated. Thank you researchers.

25 March 2020 4,352 22 View

Where can I get a free raster layer of rock-outcrops/rocky/kopjes for Tanzania/Africa?

Hi everyone I want to do species distribution modelling (SDM) for pancake tortoise in Tanzania. I understand that, pancake tortoise inhabits area with rock-outcrops such as rocky area and kopjes...

19 February 2020 283 5 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View