The challenging part of this is that once a tumor has collapsed, presumably infiltrating neutrophils will "mop up" and look like granulocytic MDSC's i.e. ly6c mid, ly6g hi, cd11b+.
Hi, first it's important to specify if you are looking mouse or human specimens. It will change the analysis.
You could look at
A Novel Ly6C/Ly6G-Based Strategy to Analyze the Mouse Splenic Myeloid Compartment
Shawn Rose, 1,2 Alexander Misharin,1,2 Harris Perlman1,2*
or
Nature. 2007 Dec 6;450(7171):825-31.
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Anthony, isn't it suspicious that the phenotype for "granulocytic MDSC" and neutrophils is essentially identical? Just call a spade a space. The entire MDSC field is based on few ancient papers with poorly done flow cytometry using Gr-1 antibody. There is no genetic evidence that it is a separate unique lineage. "Left shift", appearance of the band forms of the neutrophils (which express higher levels of Ly6C and lower of Ly6G) and even seeding of the myeloid progenitors to the tumor-bearing organ are well-known phenomena. Properly designed flow panels will help you to clearly identify proper cell types.
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