How can i stimulate Jurkat cells to differentiate into Th17 cells?

29 February 2016 1 5K Report

I learned PMA-ionomycin can use this procedure but i don't know whole process. How many days do i incubate jurkat cells and what are other chemichals that i need?

Thanks

Amarendra Varma

You cannot, because of the following reasons...

1) You are dealing with Jurkats

2) You want to use PMA/Iono for this purpose

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