How to immunofluorescence staining of the Kinetoehore on the microtubules of the oocyte
This paper with the protocol is attached.
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
Hi, Could someone explain the primary differences between deep-seated landslides and slope destabilization? In particular, definition and characteristics, mechanisms and triggering factors,...
02 August 2024 4,212 2 View
How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions?
25 July 2024 5,208 2 View
I want to study the thermal properties of a mixed system which is constructed by virtual crystal approximation in VASP. When I try to run the ab initio Molecular Dynamics of this system in VASP, I...
19 July 2024 6,569 3 View
If I want to invent my own hypothesis testing method, where should I get started ?
15 July 2024 5,376 5 View
I am currently working on a research paper focused on the control of Connected and Autonomous Vehicles (CAVs) utilizing multi-agent reinforcement learning methods. At this stage, I am seeking a...
14 July 2024 2,620 2 View
I've earned 1 best paper award and 4 best oral presentation awards. What should I do next to elevate my academic capabilities to the next level ?
14 July 2024 6,071 5 View
I read several information security books. How do I start writing anti-virus softwares ?
13 July 2024 8,180 1 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
During the heavy warm rolling process, austenite undergoes dynamic recrystallization (either DDRX or CDRX), and then transforms into martensite upon quenching. Is there a way to distinguish from...
11 July 2024 7,602 1 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View