Carlos,

We use poly-l-lysine charged coverslips to attach live cells to coverslips.

We diluted it 10 fold in D-H20, submerge the coveslips for 5 min, rinse for 5 min, drain and add live cell suspensions for 5 min.  Then we add the fixative slowly to the dish (containing the coverslip).  Good Luck

Thanks for the tips Michael!

You can cause them to differentiate by adding DMSO, after which they will become neutrophil-like cells and adhere to the coverslip for IF.

Depending on the type of fluorescence assay you can do cytospins. Alternatively, you also can do fluorescence labelling in suspension, finally resuspending the cell pellet in mounting medium.

All good answers. I have not tried HL-60, but we did something similar with THP-1, a monocyte cell line, which are also in suspension. With these cells, you can use phorbol miristate acetate (PMA), which induces differentiation to macrophage like cells and they become adherent. Maybe you could have a read to see if PMA is also used with HL-60? also, the lysine coated coverslips that Michael suggested are very helpful. Good luck

Indeed, 8-day differentiation using 1.5% DMSO, followed by activation of their by PMA (~ 3 hours, 50 nM). But get ready for NETosis in all its glory :)