I would like to develop LFIA where I am trying to use mobile phone camera or any android software as a detector.
My palsmid is around 9000 bp in pSectag2-MycHis-IRES-EGFP vector with ampicillin resistance and use DH-alpha E.coli.. It is highcopy plasmid. I tried both large and small volume of culture medium....
09 October 2018 2,546 5 View
I am trying to transfect S2 cell by pMT-Bip-GFP vector. I tried several time using cellfectine reagent and also use Mirus Trans-IT reagents. but did not succeed. Can anyone help me?
09 October 2017 5,142 3 View
09 October 2016 7,024 1 View
Actually I am trying to develop a new LFIA method and want to use fluoresccence to detect the analyte.
05 June 2016 9,203 2 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
24 February 2021 1,365 3 View
I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...
22 February 2021 6,386 1 View
Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...
22 February 2021 3,653 11 View