I have mouse brain (cortex and hippocampus) which has been been put in mPER and stored at -20. I would like to extract protein to run an immunoassay. Would anyone have a protocol for this.
Thank you very much- the N-PER sounds very good but unfortunately I already have the tissue in m-PER. I think I will try to homogenize the samples and look at the protein yield. Or would you recommend getting the N-PER and homogenizing in that?