I´m using the Reliaprep gDNA tissue Miniprep System from Promega.
I want to know if I should wash the sample or leave it in a buffer or distilled water to wash out the ethanol before using the kit.
cut up to 20of tissue and transfer into a microcentrifuge tube and add 200 microliter of buffer TL.
I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...
01 March 2021 5,902 3 View
I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...
28 February 2021 3,637 2 View
Hi everyone I'm currently working with a membrane transporter protein to which I need to simulate molecular dynamics (MD). For this, I have inserted my protein in a membrane with CHARMM-GUI and...
16 December 2020 407 1 View
We are investigating the acceptance of jellyfish-based food dishes in Latin America. If you are interested in the topic, please fill out the anonymous survey at the following link. Thanks in...
06 December 2020 4,266 2 View
Hi. I'm working with soil enzymes and I have an issue because i saw several papers that used "integrated biological response" or IBRv2 to integrate data from enzymes and build a hexagonal star...
11 October 2020 5,131 3 View
Dear community I have a great doubt related to pH in grains. It has been reported that some amino acids could be influencing the pH level. However, could minerals located in the inner parts of...
24 September 2020 6,694 14 View
I'm trying to add the generated distribution graphs to my assay but I cannot find anywhere I can do so. I thought about using the command Line built in, however nothing on the internet nor their...
14 July 2020 2,363 3 View
03 June 2020 3,361 1 View
Psychological processes as: perception, memory, learning, emotion or motivation?
26 April 2020 7,348 11 View
Can I do a path analysis with AMOS using factor scores as observable variables? Can I build a model with latent variables from observable variables derived from factorial scores? Is this...
20 February 2020 7,441 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View