I´m using the Reliaprep gDNA tissue Miniprep System from Promega.
I want to know if I should wash the sample or leave it in a buffer or distilled water to wash out the ethanol before using the kit.
cut up to 20of tissue and transfer into a microcentrifuge tube and add 200 microliter of buffer TL.
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View
I'm working on the theoretical framework of my thesis and I can't find literature to support the idea that at different floor heights, there may be differences in energy demand. If someone can...
25 March 2024 4,998 1 View
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29 February 2024 3,822 6 View
I have recently commenced research involving sulfate-reducing bacteria. For my experiments, I prepared an anoxic freshwater medium supplemented with trace elements, a vitamin mixture, and sodium...
29 January 2024 4,306 2 View
Hello, I would like to perform a western blot to detect a protein fused to GR (Glucocorticoid Receptor) and expressed from a 35S promoter in transgenic apple plants. I have never used anti-GR...
17 January 2024 7,806 0 View
Hi everybody. I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation. For...
16 January 2024 8,821 4 View
I`m trying to prepare a solution of water-cmc with the highest content of CMC possible for further processing, but I wanted to know how I can lower the viscosity so it will be easier to handle but...
04 January 2024 2,062 6 View
The liver sections look fragmented, how could the histological technique be improved to better observe this tissue under the microscope?
06 December 2023 7,388 3 View
During my immunohistochemistry procedure, after applying the chromogen diaminobenzidine, I mistakenly added other reagents—PBS, Triton, avidin, and biotin—resulting in chromogen precipitation and...
30 November 2023 6,702 1 View
Hi, I would like to mark the tip location of a NeurNexus probe. I know a good way to easily find the electrode location is to anesthetize the animal, perform an electrolytic lesion, wait a few...
21 October 2023 1,656 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View