Hi,
I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae.
Thank you very much!!!
Francesco
Hi Francesco
In our lab we did a colaboration with a facility that performed the protocol as above described:
Adipose tissue (~1 mm3) was fixed with 2% glutaraldehyde for 2 h,
followed by 1% osmium tetroxide for 2 h and 0.5% uranyl acetate over-
night. After dehydration and embedding in resin, ultra-thin sections
(90 nm) were stained with uranyl acetate and lead citrate and analyzed
in JEOL 1010 transmission electron microscope.
You can check the images in the paper:
http://www.sciencedirect.com/science/article/pii/S1388198116300506
Thiago
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