you can detect the concentration indirectly if you now its function!

Hi  Heba

You can by radiolabelling of the thymoquinone  TQ...you can also encapsulate the thymoquinine in nanoparticles or into liposomes for delivery to specific tissue and then measure the biodistribution

Radiolabeling protocol:

The TQ solution and TQ-NP1 were radiolabeled using technetium (99mTc) by a direct labeling method.17,25 One milliliter of the TQ solution and TQ-NP1 (5 mg/mL) was taken separately and stannous chloride dihydrate solution (100 mg in 100 mL of 0.10 N HCl) was added. The pH was adjusted to 7.0 ± 0.50 using 50 mM sodium bicarbonate solution. To the resultant mixture, 1 mL of sterile 99mTc-pertechnetate (75 to 400 MBq) was added gradually over a period of 1 minute with continuous mixing. The resultant mixture was incubated (30°C ± 0.5°C) for 30 minutes in an inert environment. The final volume was made up using isotonic (0.90% w/v) saline solution. The radiochemical purity of 99mTc-TQ solution (99mTc-labeled TQ) and 99mTc-TQ-NP1 (99mTc-labeled TQ-loaded CS NP) were determined by instant thin-layer chromatography (ITLC; Gelman Sciences, Inc, Ann Arbor, MI) using a previously optimized mobile phase consisting of acetone (100% v/v). The effect of incubation time, pH, and stannous chloride concentration on radiolabeling efficiency were studied to achieve optimum reaction conditions. The optimized radiolabeled formulations were assessed for in vitro stability in normal saline solution, rat plasma and in rat brain homogenate. Finally, the optimized stable radiolabeled formulations were used to study biodistribution in rats.

This is the publication Dr. Heba

And the publication for the liposome-based  delivery method is:

Thanks Dr. Amira a lot :-)