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Questions related to Histological Techniques
I need coronal sections of the substania nigra and VTA for confocal fluorescent imaging. When cutting in the brain on the cryostat, what are some useful landmarks I can use (looking grossly) to...
02 February 2016 3,353 3 View
I've stained trichodinids & sessile ciliophorans for many years using Mayer's & Harris's haematoxylin successfully. Lately a few batches of stains destained few months after initial stain....
02 February 2016 7,441 2 View
Hi everyone, I'm very interested in studying mouse subventricular zone (SVZ) sections. According to literature, vibratome cutting provides the best approach possible to observe this structure. As...
02 February 2016 2,378 2 View
I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...
02 February 2016 3,002 17 View
I am trying to measure calcium levels in hippocampus rat slices, specifically in CA1 layer, using membrane-permeable Fura-2. I am using control slices incubated without Fura-2 but with DMSO, the...
02 February 2016 7,668 4 View
Hi all scientists here in this group-help needed to find out how to count bacteria (green/red) in an image from fluorescence microscope? I have green and red bactria to count from images, for...
01 February 2016 1,657 1 View
We culture cells in 8 chamber slides and do immunofluorescent staining. We put one drop of mounting media on each of the 8 wells and drop the coverslip at a 45 degree angle. Does anyone have tips...
19 January 2016 9,084 4 View
I'd like to make an immunohistochemistry on human samples and find the kupffer cells. How can I do that? Thank you!
18 January 2016 7,766 3 View
Can I use multiple donors for T cell assays? Will mixing cause unwanted stimulation?Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to...
17 January 2016 3,856 3 View
Samples are fixed, embedded and frozen following a standard protocol though when sectioned at 10 um the tissue is flimsy, delicate, and missing sections.
11 January 2016 3,888 2 View
I wish to prepare cryosections of spheroids, do you have any protocol for OCT embedding (How many spheroids? how keep in the center of the holder, etc)? could you suggest me also some providers...
07 January 2016 10,096 4 View
I need to perform H&E stainig for the OCT embedded zebrafish brain.. Tissue section thikness is 10 micron
06 January 2016 9,893 6 View
Cell culture Biotechnology What is the range of concentration and exposure time of PFA for fixing of mammalian cells with 96 well plate?
04 January 2016 334 3 View
Im preparing E. coli and Klebsiella pneumoniae samples for SEM and I read about ethanol series but no one explain it in details. How long shall I put each sample in the series?
03 January 2016 9,209 2 View
01 January 2016 9,108 3 View
I am trying to histologically slice rat lungs. I am trying to mount either in OCT or paraffin but am looking for something to fix the tissue but also inflate the tissue. Previously, I have used to...
01 January 2016 1,899 3 View
I'm optimising a monoclonal CCL2 ab to be used in formalin fixed paraffin embedded OSCC biopsies. I don't know what can be used as positive control.
01 January 2016 2,578 2 View
I am planning to do an in situ to determine if a gene of interest is expressed in the intestine. I have done in situs in larval zebrafish but never in adults/adult tissue. Can anyone point me...
01 January 2016 8,720 7 View
01 January 2016 9,859 4 View
Dear All, I’m preparing the total cell suspension from the human lung tissue; that contains macrophages, DCs and alveolar epithelial cells. I want to isolate the resident lung macrophages from the...
01 January 2016 873 1 View
We are staining mouse liver sections, the detection system is HRP-DAB. The pictures are looking good but you know how it is, the signal could always be a bit stronger. I'd like to hear from people...
01 January 2016 4,135 6 View
The waterpik generates large amounts of water making it difficult to freeze small amounts of samples in the field. How do I remove only the tissue and symbionts of coral while preserving the...
01 January 2016 4,958 2 View
I need to confirm if my THP1 cell differentiation into macrophages worked. I'm planning to use CD11b and CD14 for staining (fluorescence microscopy). Do we have to block Fc antibodies in both...
01 January 2016 3,658 2 View
01 January 2016 7,923 3 View