I am collecting embryos for histological analysis and need to genotype them beforehand. In the past I have worked with older mouse embryos (E8.5 and up), where it is easy to remove the yolk sac and use it for genotyping. However, I now need to start collecting E6.5 embryos. Given that they are so small, I am not sure whether there is enough material to be able to save the epiblast for histology and use other tissue for genotyping. Does anyone have experience with genotyping E6.5 embyros?
Hello
Article and link here will help you
Good Luck
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC316346/
Article Oocyte-derived Smad4 is not required for development of the ...
Hello Margot,
I did paraffin sectioning of 7.5 and I used to genotype the residual tissue (endoderm around the node) after sectioning. I took a tiny piece directly from the paraffin block using tweezers under binocular. I isolated the DNA for genotyping using 5h digest in 30ul of Fast tissue lysis buffer with 3ul ProteinaseK, took 2,5ul and run the PCR in 20ul of https://www.thermofisher.com/order/catalog/product/K1081.
Hope it helps,
Cheers,
Jan
Article Oocyte-derived Smad4 is not required for development of the ...
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