Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
Hai, I am using the concept of filling liquids/gels in the matrix of a photonic crystal structure. How can we calculate the effective refractive index of such structures? Thanks in advance....
01 January 2020 8,057 3 View
I ran an SDS-PAGE gel of my total cell samples, each expressed in increasing concentrations of a co-factor to see whether this improves expression of the target protein. I coomassie stained the...
01 January 2020 8,348 3 View
I found too many bands when ~30 microgram protein loaded in the gel from cancer cell lines, but I see very clean band in normal cells! Your precious comments will be appreciated! Thanks
01 January 2020 7,473 3 View
I've prepared lipid(DOPC) GUVs(giant vesicles) in 100mM glucose in PBS solution by gel-assisted method. And in order to harvest them I pipette the solution so they can be peeled off from the gel...
01 January 2020 9,458 2 View
The shift it's working, but the RNA band is not very stable, sometimes it appears, sometimes not. And at the wells with a higher concentration of protein, I still see a big RNA band. I think maybe...
01 January 2020 2,435 2 View
I'm trying to run a Northern blot assay on collagen pads, but have had difficulty dissolving the samples. I have tried using 0.01M and 1M HCl as a solvent with only partial success (the samples...
01 January 2020 6,227 3 View
while making sol - gel , for spin coating deposition of TiO2 i m not getting the transparent gel , how an i get it , white precipitate are forming very soon ?
01 January 2020 9,273 5 View
I was watching a video about self-siphoning poly(ethylene glycol) gels. Then I found that typically these PEGs have a molecular weight over a million. I was wondering why the PEGs with such high...
01 January 2020 8,617 3 View
Hello everyone, I have been having problems with insertion of my gene (195bp long coding sequence) into pGBKT7 using Gateway technology. I always perform LR reaction (incubating for at least 1...
01 January 2020 214 3 View
Several herbs are used by traditional healers and it is proofed to be higly effective for trearing various dermatological disease. In your opinion what are the recommended steps to market these...
01 January 2020 2,927 3 View
I m making 10 percent seperating gel but my both gel stacking and resolving are not solidify plz let me know how to resolve this issue
01 January 2020 5,223 3 View
Hi there, I hope you are doing well. I am working with imaging systems. I am confused about the effects of linear polarizer in such systems ( I mean how a linear polarizer can improve the...
22 December 2019 5,383 6 View
I used Y-PER protein extraction reagent to extract yeast protein. Then, I quantified the amount of protein by BCA. The problem is when I using this protein to run SDS-PAGE, the loading dye in gel...
12 December 2019 9,127 3 View
I am currently trying to troubleshoot why my gels are not showing anything at all except for my ladder marker bands. I have attached an example of a gel that I used to run previously with no...
12 December 2019 6,593 4 View
Hello to everyone, I'm trying to amplify nuclear markers from old museum specimens of beetle (not very old, sampled 10-15 years ago) , I thought on the Wingless fragment, it's obviously a hard...
12 December 2019 5,690 3 View
Few minutes after adding PBS 1x to wash 3D printed scaffolds, the gel got all dissolved. Loads of papers use PBS to wash it and apparently they did not have any problem. Any advice? Did anyone has...
12 December 2019 2,777 2 View
I am new to SDS-PAGE and hope someone can help with a persistent problem I am experiencing. The dye front is stopping about 3/4 of the way down the gel and doesn't seem to move further down than...
12 December 2019 9,919 3 View
I try to produce a Chitosan Hydrogel that would be bioprinted, I made this by making a 1% chitosan and mixing with 5% gelation and then cross linking with Sodium hydroxide thereby using the...
12 December 2019 6,327 5 View
Is it important to fill empty wells of SDS gel while running western blot? What can we load in empty wells?
12 December 2019 6,668 3 View
The ultrasound gel which is commercially available contains lot of air bubbles.Which creates lot of noise while scanning. How to avoid the air bubbles in the gel such that the scan wont have much...
12 December 2019 9,933 2 View
I'm making interactions between two proteins of similar molecular weights (30 kDa & 31 kDa). Is it possible to identify these two proteins (after interaction) on a gel?
12 December 2019 5,175 3 View
I did my PCR and ran the gel on the second day, I saw a very faint band ( that is more r less invicible) and this also include the DNA ladder, what could be the problem. I have used this ladder...
12 December 2019 4,778 3 View
I am conducting hemi-nested One-Step PCR assay on rectal swabs from bats stored in RNAlater. In the first 300 samples, I got clear and beautiful gel. Recently, the gel picture became as the...
12 December 2019 268 3 View
Dear Colleagues I have recently run several 12% SDS-PAGE gels. I have loaded varying protein concentrations, ranging from 2.0 to 3.0mg/ml. I have stained the gels with Comassie blue. Would you...
12 December 2019 2,248 3 View