Zymogram or native gel for amylase enzyme?

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Where to download DEM from 2018?

I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...

31 July 2024 9,757 3 View

Human health risk assessment of ambient air pollutants, how to calculate hazard quotient?

I have calculated CDI (inhalation) of Ozone, CO, SO2, NO2, NO, and TSP (total suspended particulate matter) . However I cannot find RfC (Reference concentration) or even NOAEL of any of these...

27 June 2024 4,488 0 View

Thesis For BBA Students?

Hi, My name is Muhammad Afnan Tariq. I'm 6th semester student at university in pakistan and currently enrolled in BBA Program. I want to write a thesis as fyp. I have interest in Marketing and...

23 June 2024 9,160 3 View

Is the Moderator variable different from the mediating variable?

11 June 2024 7,855 3 View

In a water balance computation, Which is better to use GETFLOWS or FEFLOW Model?

I mean in surface, evapotranspiration and groundwater interaction

04 June 2024 9,478 0 View

Are You Interested in Collaborating on Chemistry Research?

Hello ResearchGate Community, I am an MS Chemistry student with a keen interest in advancing my research skills and contributing to impactful projects. As I embark on my journey in the world of...

17 May 2024 3,213 4 View

What unique physiological traits do wild BSF adults exhibit?

differences in physiological traits of wild and domesticated (controlled/Lab Reared) black soldier fly

04 May 2024 173 0 View

Requesting Information regarding Damask Convergence Criteria?

Hi Everyone H am working on damask to run the simulation for phenomenological crystal plasticity spectral solver. Actually one problem i am facing regarding the convergence criteria in damask, i...

22 April 2024 8,179 0 View

What are necessary key point one should consider during subunit computational (MEV) vaccine construction?

Clarify a point for Multiepitope vaccine construction that 1) one should construct 3D structure of MEV with protein adjuvant sequence (A.A) or without it. 2)3D structure refinement, validation,...

06 April 2024 9,893 2 View

Nanomaterial synthesis Bottom-up /Chemical etching ?

what is the procedure of synthesis of nanomaterial -Bottom-up -Chemical Etching? Advantage and disadvantagesAdvantages of this technique?

04 April 2024 2,522 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View