What is are the different classifications of a tool changer?

I have roamed the internet trying to see the different types of tool changers available but I found no definitive answers, could you kindly help me?

02 February 2021 5,036 1 View

Hi, does any one know how to redisperse the ppt of gold NPs formed after conjugation with the drug molecule?

gold surface is already conjugated with the PEG-2000 linker but still when I tried to conjugate it with the drug, It gets precipitated

25 January 2021 2,602 1 View

How to calculate covariance of Expectation Maximization Clustering?

I am working with MatLab R2017a, I have to implement EM clustering. But I am stuck with initialization of covariance in it. I have to calculate covariance using the dataset which is comprised of...

25 December 2020 4,620 3 View

MatLab code for Expected Maximization clustering of n-Dimensional data?

I need matlab code for expected maximization clustering of n-Dimensional data. I have searched and found code related to 1-D and 2-D. Can someone refer the code for n-D .

22 December 2020 5,110 3 View

Where can I find papers related to microstructure of inconel alloy 800?

I need to define the microstructure of inconel 800, but I cannot find any literature related to it. Literature is about incoloy 800 mostly which is a bit different. Can anyone tell me if there...

07 December 2020 3,137 3 View

Do anyone have any authentic material regarding Cultural Ethics and especially regarding Pakistani culture?

I need this because I have to represent it in a conference as a keynote speaker

17 November 2020 584 4 View

Which software application is best for Anova analysis?

I want to perform "ANOVA" analysis of three different vegetation indices. anyone know how can i perform and which application /software are suitable for this. Thank you in advance.

16 November 2020 2,599 4 View

How can I specify the parameters for nano creep?

Which parameters should I employ for nano creep testing of Titanium and Inconel alloys? Should I load it with a constant load rate or strain rate? What should be the maximum load? What should be...

29 October 2020 5,727 4 View

How to find the elastic work using nanoindentation load depth curves?

I am using work of indentation approach to find out the values of hardness of Ti and Inconel alloys using nanoindentation load-depth curves. I can find the formula for total work. Whereas for...

13 October 2020 2,778 5 View

How to measure the volume of hydrogel?

08 October 2020 2,745 9 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View