In other words, given the gel percentage, voltage, protein size, buffer concentration, can you predict how fast a protein or DNA segment should theoretically travel down the gel?
A related question is: do protein samples run at constant acceleration down the gel (assuming constant pH)?
Yuri Mirgorod
For ideal prediction according to the "theory of plate", an ideal technology for the distribution of matter between the stationary and mobile phases is required. There should be an equilibrium distribution, the equilibrium constant for your eluent is known ... In fact, equilibrium in each section of the column is not achieved. In addition, the distribution depends on the analyte, the eluent.
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